Feil V J, Huwe J K, Zaylskie R G, Davison K L, Anderson V L, Marchello M, Tiernan T O
Biosciences Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Fargo, North Dakota 58105-5674, USA.
J Agric Food Chem. 2000 Dec;48(12):6163-73. doi: 10.1021/jf0003092.
Four calves were fed polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans for 120 days at levels somewhat higher than what may be found in forage near some waste incinerators and manufacturing plants. Four calves were fed identical diets but without the chemicals. Using bioelectrical impedance measurements of total body fat, 30-50% of the dosed 2,3,7,8-TCDD, 1,2,3,7,8-PeCDD, and 2,3,4,7,8-PeCDF was estimated to be retained by the animals. Although these same congeners were bioconcentrated in adipose tissue (BCF approximately 10), consumer products such as ribeye showed concentrations less than what were found in the animal feed (BCF approximately 0.1). Distribution of the dioxins and furans into various lipid compartments appeared to be rather uniform in back fat, perirenal fat, and ribeye for tetra to hexa congeners. Ribeye, serum, and liver lipids had higher concentrations of the higher chlorinated congeners, due in part to not reaching a steady state. An unexpected source of dioxin and furan contamination was discovered during the experiment, resulting in the control animals having concentrations of some congeners that were equal to or in some cases greater than those of the dosed animals. Pentachlorophenol-treated wood components in the pole barn where the feeding experiment was conducted were found to have contributed to the animals' exposure.
四头小牛被喂食多氯二苯并对二噁英和多氯二苯并呋喃，持续120天，其剂量略高于在一些垃圾焚烧炉和制造工厂附近的草料中可能发现的含量。另外四头小牛喂食相同的饲料，但不含这些化学物质。通过生物电阻抗测量全身脂肪，估计给药的2,3,7,8 - 四氯二苯并对二噁英、1,2,3,7,8 - 五氯二苯并对二噁英和2,3,4,7,8 - 五氯二苯并呋喃中有30 - 50%被动物留存。尽管这些同系物在脂肪组织中生物浓缩（生物浓缩系数约为10），但像肋眼牛排这样的消费品中显示的浓度低于动物饲料中的浓度（生物浓缩系数约为0.1）。对于四至六氯同系物，二噁英和呋喃在各种脂质隔室中的分布在背膘、肾周脂肪和肋眼牛排中似乎相当均匀。肋眼牛排、血清和肝脏脂质中高氯代同系物的浓度较高，部分原因是未达到稳态。在实验过程中发现了二噁英和呋喃污染的一个意外来源，导致对照动物某些同系物的浓度等于或在某些情况下高于给药动物的浓度。在进行饲养实验的杆式畜舍中，发现经五氯苯酚处理的木材部件导致了动物的暴露。
