Vasil M L, Holmes R K, Finkelstein R A
Science. 1975 Mar 7;187(4179):849-50. doi: 10.1126/science.1114331.
Matings between strains of Vibrio cholerae differing in toxinogenicity, nutritional requirements, and antibiotic susceptibilities were performed in order to determine the location of the gene tox that controls production of cholera enterotoxin. Segregation analysis shows that tox is linked to a gene required for histidine biosynthesis. Our data indicate that the tox gene is located on the bacterial chromosome and not on a plasmid in the strains of V. cholerae studied.
为了确定控制霍乱肠毒素产生的毒素基因（tox）的位置，对霍乱弧菌不同菌株进行了交配实验，这些菌株在产毒性、营养需求和抗生素敏感性方面存在差异。分离分析表明，tox基因与组氨酸生物合成所需的一个基因相连。我们的数据表明，在所研究的霍乱弧菌菌株中，tox基因位于细菌染色体上，而不是质粒上。
