Newman D, Tucci M, Puckett A, Hughes J, Benghuzzi H
University of Mississippi Medical Center, Jackson, MS 39216, USA.
Biomed Sci Instrum. 1999;35:229-34.
The use of homopolymers as coatings for biomaterials has received much attention in the last decade. However, the modifications and alterations induced by using such materials towards inflammatory cells have not been fully investigated. The specific objectives of this study were to investigate the role of hetero and homopolymers of amino acids on cell proliferation, and to determine the biochemical behavior associated with the incubation of RAW cells with various polymers. The RAW macrophage cell line was obtained from the American Type Culture Collection (Rockville, MD) and maintained in sterile media (RPMI) supplemented with 10% fetal bovine serum and 1% antibiotics and antimycotics. The cells were plated at a density of 1 x 10(6) cells/ml onto 24 well plates. The plates were divided into four groups of six wells per group per phase (24, 48, and 72 hours). Cells in the first group were treated with RGD, cells in group II were treated with poly-L-lysine, group III cells were treated with RGD + poly-L-lysine, and cells in group IV were treated with media alone, and served as controls. Cell number, as well as, protein, MDA, lactate dehydrogenase (LDH), and cytochrome C (cyto C) were determined at the end of 24, 48 and 72 hours. Data obtained from this investigation revealed that: (I) there were no significant difference in total cell counts between all experimental groups and control at the end of the 48 hour phase. However, at 24 hours there were fewer cells in the poly-L-lysine treated wells in comparison to control group (p < 0.05), (II) RGD and poly-L-lysine treatments did not cause changes in MDA or protein concentrations for the entire duration of the experiment, and (III) RGD treatment for 48 and 72 hours did not cause a reduction in the LDH activity compared to control and poly-L-lysine treated groups. Data obtained from this investigation could provide more insight regarding the design and development for safe and biocompatible orthopedic, dental and drug delivery devices.
在过去十年中，使用均聚物作为生物材料涂层受到了广泛关注。然而，使用此类材料对炎症细胞产生的修饰和改变尚未得到充分研究。本研究的具体目标是研究氨基酸的杂聚物和均聚物对细胞增殖的作用，并确定与RAW细胞与各种聚合物孵育相关的生化行为。RAW巨噬细胞系购自美国典型培养物保藏中心（马里兰州罗克维尔），并在补充有10%胎牛血清和1%抗生素及抗真菌剂的无菌培养基（RPMI）中培养。将细胞以1×10(6)个细胞/毫升的密度接种到24孔板中。每个阶段（24、48和72小时）将板分为四组，每组六个孔。第一组细胞用RGD处理，第二组细胞用聚-L-赖氨酸处理，第三组细胞用RGD +聚-L-赖氨酸处理，第四组细胞仅用培养基处理，作为对照。在24、48和72小时结束时测定细胞数量以及蛋白质、丙二醛（MDA）、乳酸脱氢酶（LDH）和细胞色素C（细胞色素C）。从本研究中获得的数据表明：（I）在48小时阶段结束时，所有实验组与对照组之间的总细胞计数没有显著差异。然而，在24小时时，与对照组相比，聚-L-赖氨酸处理的孔中的细胞较少（p < 0.05），（II）在整个实验期间，RGD和聚-L-赖氨酸处理不会导致MDA或蛋白质浓度的变化，并且（III）与对照组和聚-L-赖氨酸处理组相比，RGD处理48和72小时不会导致LDH活性降低。从本研究中获得的数据可以为安全且生物相容的骨科、牙科和药物递送装置的设计和开发提供更多见解。
