Thrash A, Puckett A, Tucci M, Read K, Roberts B, Benghuzzi H
University of Mississippi Medical Center, Jackson, MS 39216, USA.
Biomed Sci Instrum. 1999;35:93-8.
The use of glass ionomers as a novel bone cement is currently being investigated. Although acceptable for use as a dental restorative material, there is little information regarding how ionomers interact with inflammatory macrophage cell types. The specific objective of this experiment was to investigate the possible interrelationship between RAW and human monocyte/macrophage cells at the biochemical and morphological level after being in contact with three different dental cement ionomers (Fuji Duet, Fuji IX, and GC-Fuji-Ortho, GC America Inc., Chicago IL) for 72 hours. Transformed RAW macrophages were obtained from the American Type Culture collection (ATCC), and the non-transformed human macrophages were obtained from the peripheral blood of 25 male and female volunteers. The cells were plated at a density of 4 x 10(6) cells/ml in twenty-four well plates. Each plate was divided into four groups of six cells/group. Twenty-four hours after plating, the cells in groups I-III were incubated with Fuji Duet, Fuji IX, GC Fuji Ortho, respectively, and cells in Group IV were incubated with media alone to serve as controls. Immediately after addition of the ionomers, the cellular morphology was monitored for both transformed and non-transformed cells. Cell number data revealed that normal non-transformed cells were similar in number to control cells in media alone. This result suggests that the polymer treatment did significantly alter cellular viability. On the other hand, RAW cell number was markedly reduced in cells treated with ionomers in comparison to cell growing in media alone. The data suggests that the prescence of the ionomer may reduce the proliferation rate of RAW cells. Biochemical analysis of cellular supernatants to determine cellular alterations at 72 hours revealed increased levels of lactate dehydrogenase activity and levels of malionaldehyde bis diethyl acetal in all ionomer-treated groups of RAW cells compared with media alone. Non-transformed macrophages treated with the same ionomers did not differ significantly from the control cells in media alone. However, when comparing the levels of lactate dehydrogenase activity between the transformed and non-transformed cells it was apparent that the normal cells exhibited statistically higher activity than the RAW transformed cells. The results of this study suggest that although the three ionomers tested were found to be highly biocompatible with fully differentiated non-transformed macrophages, the behavior of transformed and non-transformed phagocytic cells towards these ionomers may not be similar under similar conditions.
目前正在研究将玻璃离子聚合物用作新型骨水泥。尽管玻璃离子聚合物作为牙科修复材料是可以接受的，但关于离子聚合物如何与炎症巨噬细胞类型相互作用的信息却很少。本实验的具体目的是研究RAW细胞和人单核细胞/巨噬细胞在与三种不同的牙科粘固剂离子聚合物（Fuji Duet、Fuji IX和GC-Fuji-Ortho，GC America Inc.，芝加哥，伊利诺伊州）接触72小时后，在生化和形态学水平上可能存在的相互关系。转化的RAW巨噬细胞购自美国典型培养物保藏中心（ATCC），未转化的人巨噬细胞取自25名男性和女性志愿者的外周血。将细胞以4×10⁶个细胞/毫升的密度接种于24孔板中。每块板分为四组，每组六个细胞。接种24小时后，第I-III组的细胞分别与Fuji Duet、Fuji IX、GC Fuji Ortho一起孵育，第IV组的细胞仅与培养基一起孵育作为对照。在添加离子聚合物后，立即监测转化细胞和未转化细胞的细胞形态。细胞数量数据显示，正常未转化细胞的数量与仅在培养基中的对照细胞相似。这一结果表明聚合物处理并未显著改变细胞活力。另一方面，与仅在培养基中生长的细胞相比，用离子聚合物处理的RAW细胞数量明显减少。数据表明离子聚合物的存在可能会降低RAW细胞的增殖率。对细胞上清液进行生化分析以确定72小时时的细胞变化，结果显示与仅培养基相比，所有用离子聚合物处理的RAW细胞组中乳酸脱氢酶活性水平和丙二醛双二乙缩醛水平均升高。用相同离子聚合物处理的未转化巨噬细胞与仅在培养基中的对照细胞相比无显著差异。然而，当比较转化细胞和未转化细胞之间的乳酸脱氢酶活性水平时，很明显正常细胞的活性在统计学上高于RAW转化细胞。本研究结果表明，尽管所测试的三种离子聚合物被发现与完全分化的未转化巨噬细胞具有高度生物相容性，但在相似条件下，转化和未转化的吞噬细胞对这些离子聚合物的行为可能并不相似。
