Johnston W, Carr S, Benghuzzi H, Tucci M, Puckett A, Tsao A, Hughes J
School of Health Related Professions, Department of Orthopaedic Surgery and School of Dentistry, University of Mississippi Medical Center, Jackson, MS 39216, USA.
Biomed Sci Instrum. 1999;35:217-22.
Upon introduction into the human body, biomaterials initially trigger a foreign-body inflammatory response. Furthermore, the wear debris associated with such materials as those used for orthopedic implants, artificial heart valves, and dental implants can cause the body to mount an inflammatory response. This involves the production of phagocytic macrophages that ingest the foreign material while simultaneously producing cytokines that serve as chemotactic agents for an amplified immune response. Currently titanium (Ti), polyethylene (PE), tricalcium phosphate (TCP) and hydroxyapatite (HA) are widely used as biomaterials in medical implants, and particle size is an important factor in the development of orthopedic, dental, and medical implants. The objective of this study was to investigate the effect of various biomaterials (Ti, mixed particle size polyethylene (MPE), ultra high molecular weight polyethylene (UHMWPE), mixed particle size TCP (MTCP), < 0.38 micron TCP (S-TCP), and hydroxyapatite (HA)) on the inflammatory reactions expressed by transformed RAW macrophages. RAW transformed monocytes were obtained from the American Cell Culture Line, (Rockville, MD). The cells were allowed to incubate in contact with the materials for intervals of 24, 48, and 72 hours. Biochemical tests and morphological evaluations were performed after each time point, including screening for cell number, cell protein levels, supernatant protein levels, lactate dehydrogenase (LDH), Maliondialdehyde (MDA), catalase by following standard lab protocols.
生物材料一旦进入人体，最初会引发异物炎症反应。此外，与骨科植入物、人工心脏瓣膜和牙科植入物等材料相关的磨损碎片会导致身体产生炎症反应。这涉及吞噬性巨噬细胞的产生，这些巨噬细胞吞噬异物，同时产生细胞因子，作为放大免疫反应的趋化剂。目前，钛（Ti）、聚乙烯（PE）、磷酸三钙（TCP）和羟基磷灰石（HA）作为生物材料广泛应用于医疗植入物中，颗粒大小是骨科、牙科和医疗植入物开发中的一个重要因素。本研究的目的是调查各种生物材料（Ti、混合粒径聚乙烯（MPE）、超高分子量聚乙烯（UHMWPE）、混合粒径TCP（MTCP）、<0.38微米TCP（S-TCP）和羟基磷灰石（HA））对转化的RAW巨噬细胞表达的炎症反应的影响。RAW转化单核细胞取自美国细胞培养株（马里兰州罗克维尔）。使细胞与材料接触孵育24、48和72小时。在每个时间点后进行生化测试和形态学评估，包括按照标准实验室规程筛选细胞数量、细胞蛋白水平、上清液蛋白水平、乳酸脱氢酶（LDH）、丙二醛（MDA）、过氧化氢酶。
