Carr S, Johnston W, Benghuzzi H, Tucci M, Puckett A, Tsao A, Hughes J
School of Health Related Professions, Department of Orthopaedic Surgery And School of Dentistry, University of Mississippi Medical Center, Jackson, MS 39216, USA.
Biomed Sci Instrum. 1999;35:211-6.
Although tissue culture techniques are used extensively to explore the biocompatibility of various biomaterials used in orthopaedic, dental and pharmaceutical fields, the role of these materials towards human monocytes/macrophages has not been fully elucidated. The specific objectives of this investigation were: (1) to determine the biochemical markers resulting from exposure of the human monocytes/macrophages to titanium (TI), large size polyethylene (LSP), submicron polyethylene (SPE), hydroxyapatite (HA), large particle size tricalcium phosphate (LTCP), and small particle size tricalcium phosphate (STCP), and (2) to morphologically evaluate the viability of the cells treated with the aforementioned biomaterials. Approximately 15 volunteers donated blood for each phase (24, 48, and 72 hours) of the experiment. The monocytes were isolated by following established lab procedures (Histopaque 1077 and 1119). Aseptic techniques were followed throughout each phase. Each phase contained four experimental groups (TI, LSP, SPE, HA). Each group was comprised of six wells. The total protein, catalase, LDH, MDA, and cell count were measured using established lab protocols. Data obtained suggests that: (I) regardless of the biomaterial being used all experimental groups experienced remarkable phagocytosis in the first two phases (24, 48 hours), (II) during the 24 hour phase MDA activities were increased in TI, LTCP, and STCP treated wells when compared to the control and other experimental groups, (III) in the 48 hour phase the MDA level increased in LPE and STCP treated cells, (IV) there were significant differences in LDH levels in LPE, STCP, and SPE at 24 hours compared to the control and other experimental groups, (V) LDH activities were increased in LPE, STCP, SPE, and LTCP at 48 hours, and (VI) at 72 hours there were significant increases in catalase activity in HA, TI, SPE and LPE when compared to the control group and other experimental groups. Information obtained from this study provided new ideas about the interrelationship of various biomaterials, the effect of size and cell response to the various biomaterials.
尽管组织培养技术被广泛用于探索骨科、牙科和制药领域中各种生物材料的生物相容性，但这些材料对人类单核细胞/巨噬细胞的作用尚未完全阐明。本研究的具体目标是：（1）确定人类单核细胞/巨噬细胞暴露于钛（TI）、大尺寸聚乙烯（LSP）、亚微米聚乙烯（SPE）、羟基磷灰石（HA）、大粒径磷酸三钙（LTCP）和小粒径磷酸三钙（STCP）后产生的生化标志物，以及（2）从形态学上评估用上述生物材料处理的细胞的活力。在实验的每个阶段（24、48和72小时），约15名志愿者献血。单核细胞通过既定的实验室程序（Histopaque 1077和1119）分离。在每个阶段都遵循无菌技术。每个阶段包含四个实验组（TI、LSP、SPE、HA）。每个组由六个孔组成。使用既定的实验室方案测量总蛋白、过氧化氢酶（CAT）、乳酸脱氢酶（LDH）、丙二醛（MDA）和细胞计数。获得的数据表明：（I）无论使用何种生物材料，所有实验组在前两个阶段（24、48小时）均经历了显著的吞噬作用；（II）在24小时阶段，与对照组和其他实验组相比，TI、LTCP和STCP处理的孔中MDA活性增加；（III）在48小时阶段，LPE和STCP处理的细胞中MDA水平升高；（IV）在24小时时，LPE、STCP和SPE组的LDH水平与对照组和其他实验组相比存在显著差异；（V）在48小时时，LPE、STCP、SPE和LTCP组的LDH活性增加；（VI）在72小时时，与对照组和其他实验组相比，HA、TI、SPE和LPE组的过氧化氢酶活性显著增加。本研究获得的信息为各种生物材料之间的相互关系、尺寸效应以及细胞对各种生物材料的反应提供了新的思路。
