编码一种应激诱导伴侣蛋白的紫球海胆cDNA的分离与鉴定。
Isolation and characterization of a Paracentrotus lividus cDNA encoding a stress-inducible chaperonin.
作者信息
Gianguzza F, Ragusa M A, Roccheri M C, Di Liegro I, Rinaldi A M
机构信息
Dipartimento di Biologia Cellulare e dello Sviluppo A. Monroy, Palermo, Italy.
出版信息
Cell Stress Chaperones. 2000 Apr;5(2):87-9. doi: 10.1379/1466-1268(2000)005<0087:iacoap>2.0.co;2.
Abstract
Chaperonins are ubiquitous proteins that facilitate protein folding in an adenosine triphosphate-dependent manner. Here we report the isolation of a sea urchin cDNA (Plhsp60) coding for mitochondrial chaperonin (Cpn60), whose basal expression is further enhanced by heat shock. The described cDNA corresponds to a full-length mRNA encoding a protein of 582 amino acids, the first 32 of which constitute a putative mitochondrial targeting leader sequence. Comparative analysis has demonstrated that this protein is highly conserved in evolution.
摘要
伴侣蛋白是普遍存在的蛋白质，它们以三磷酸腺苷依赖的方式促进蛋白质折叠。在此，我们报告了一种编码线粒体伴侣蛋白（Cpn60）的海胆cDNA（Plhsp60）的分离，其基础表达在热休克后进一步增强。所述cDNA对应于一个全长mRNA，编码一个582个氨基酸的蛋白质，其中前32个氨基酸构成一个推定的线粒体靶向前导序列。比较分析表明，这种蛋白质在进化过程中高度保守。
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