人类细胞核核糖核酸酶P的一个亚基具有ATP酶活性。
A subunit of human nuclear RNase P has ATPase activity.
作者信息
Li Y, Altman S
机构信息
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):441-4. doi: 10.1073/pnas.98.2.441. Epub 2001 Jan 9.
Abstract
Human nuclear RNase P purified from HeLa cells has ATPase activity. This activity is associated with one of the protein subunits of the enzyme, Rpp20. Thus, human nuclear RNase P, which contains several proteins and one essential RNA, has at least one other enzymatic activity in addition to cleavage of phosphoester bonds in RNA. The amino acid sequence of Rpp20 has a signature motif found in an ATPase-containing subunit of a family of protein complexes (ABC transporters) that mediate a variety of trans-membrane traffic, as well as a segment, DIxxN, that resembles the DEAD box motif of many ATPases: together, these might represent an ATPase signature motif.
摘要
从人宫颈癌细胞（HeLa细胞）中纯化出的人源细胞核核糖核酸酶P具有ATP酶活性。该活性与该酶的一种蛋白质亚基Rpp20相关。因此，人源细胞核核糖核酸酶P含有多种蛋白质和一种必需RNA，除了能切割RNA中的磷酸酯键外，还至少具有一种其他酶活性。Rpp20的氨基酸序列具有一个特征基序，该基序存在于一类介导多种跨膜运输的蛋白质复合物（ABC转运蛋白）的含ATP酶亚基中，还有一段类似于许多ATP酶的DEAD盒基序的序列DIxxN：这些共同组成了一个ATP酶特征基序。
相似文献
1
A subunit of human nuclear RNase P has ATPase activity.人类细胞核核糖核酸酶P的一个亚基具有ATP酶活性。
Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):441-4. doi: 10.1073/pnas.98.2.441. Epub 2001 Jan 9.
2
Mapping RNA-protein interactions in ribonuclease P from Escherichia coli using disulfide-linked EDTA-Fe.利用二硫键连接的EDTA-铁映射大肠杆菌核糖核酸酶P中的RNA-蛋白质相互作用。
J Mol Biol. 2000 Feb 11;296(1):19-31. doi: 10.1006/jmbi.1999.3443.
3
Molecular modeling of the three-dimensional structure of the bacterial RNase P holoenzyme.细菌核糖核酸酶P全酶三维结构的分子建模
J Mol Biol. 2003 Jan 24;325(4):661-75. doi: 10.1016/s0022-2836(02)01267-6.
4
Characterization of RNase P from Thermotoga maritima.嗜热栖热菌核糖核酸酶P的特性分析
Nucleic Acids Res. 2001 Feb 15;29(4):880-5. doi: 10.1093/nar/29.4.880.
5
Bacterial ribonuclease P holoenzyme crosslinking analysis reveals protein interaction sites on the RNA subunit.细菌核糖核酸酶P全酶交联分析揭示了RNA亚基上的蛋白质相互作用位点。
Nucleic Acids Res. 2001 Sep 15;29(18):3848-56. doi: 10.1093/nar/29.18.3848.
6
Inhibition of bacterial RNase P by aminoglycoside-arginine conjugates.氨基糖苷-精氨酸缀合物对细菌核糖核酸酶P的抑制作用。
FEBS Lett. 2002 Jan 30;511(1-3):107-12. doi: 10.1016/s0014-5793(01)03322-1.
7
Cloning, purification and characterization of the protein subunit of ribonuclease P from the cyanobacterium Synechocystis sp. PCC 6803.来自集胞藻属蓝细菌PCC 6803的核糖核酸酶P蛋白亚基的克隆、纯化及特性分析
Eur J Biochem. 1996 Oct 1;241(1):17-24. doi: 10.1111/j.1432-1033.1996.0017t.x.
8
Evidence for an RNA-based catalytic mechanism in eukaryotic nuclear ribonuclease P.真核细胞核核糖核酸酶P中基于RNA的催化机制的证据。
RNA. 2000 Apr;6(4):554-62. doi: 10.1017/s1355838200991477.
9
Protein-protein interactions with subunits of human nuclear RNase P.与人类细胞核核糖核酸酶P亚基的蛋白质-蛋白质相互作用
Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):920-5. doi: 10.1073/pnas.98.3.920. Epub 2001 Jan 16.
10
Analysis of the functional role of conserved residues in the protein subunit of ribonuclease P from Escherichia coli.大肠杆菌核糖核酸酶P蛋白亚基中保守残基的功能作用分析。
J Mol Biol. 1997 Apr 11;267(4):818-29. doi: 10.1006/jmbi.1997.0906.
引用本文的文献
1
Evolution of sequence, structural and functional diversity of the ubiquitous DNA/RNA-binding Alba domain.普遍存在的DNA/RNA结合Alba结构域的序列、结构和功能多样性的演变
Sci Rep. 2024 Dec 5;14(1):30363. doi: 10.1038/s41598-024-79937-4.
2
MicroRNA750-3p Targets to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector .miR750-3p 靶向抑制介体中的水稻黑条矮缩病毒增殖。
Viruses. 2024 Jan 8;16(1):97. doi: 10.3390/v16010097.
3
RNA binding protein POP7 regulates ILF3 mRNA stability and expression to promote breast cancer progression.RNA 结合蛋白 POP7 通过调控 ILF3 mRNA 的稳定性和表达促进乳腺癌的进展。
Cancer Sci. 2022 Nov;113(11):3801-3813. doi: 10.1111/cas.15430. Epub 2022 Sep 6.
4
The Dynamic Network of RNP RNase P Subunits.RNP RNase P 亚基的动态网络。
Int J Mol Sci. 2021 Sep 24;22(19):10307. doi: 10.3390/ijms221910307.
5
Crystal Structure of Human Rpp20/Rpp25 Reveals Quaternary Level Adaptation of the Alba Scaffold as Structural Basis for Single-stranded RNA Binding.人源 Rpp20/Rpp25 复合物的晶体结构揭示了 Alba 支架的四级结构适应作为单链 RNA 结合的结构基础。
J Mol Biol. 2018 May 11;430(10):1403-1416. doi: 10.1016/j.jmb.2018.03.029. Epub 2018 Apr 4.
6
Characterization of ATPase Activity of P2RX2 Cation Channel.P2RX2阳离子通道的ATP酶活性表征
Front Physiol. 2016 May 24;7:186. doi: 10.3389/fphys.2016.00186. eCollection 2016.
7
Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III.人核糖核酸酶P核糖核蛋白是RNA聚合酶III起始复合物形成所必需的。
Nucleic Acids Res. 2015 Jun 23;43(11):5442-50. doi: 10.1093/nar/gkv447. Epub 2015 May 7.
8
Of proteins and RNA: the RNase P/MRP family.关于蛋白质和 RNA：RNase P/MRP 家族。
RNA. 2010 Sep;16(9):1725-47. doi: 10.1261/rna.2214510. Epub 2010 Jul 13.
9
Eukaryotic ribonucleases P/MRP: the crystal structure of the P3 domain.真核生物核糖核酸酶 P/MRP：P3 结构域的晶体结构。
EMBO J. 2010 Feb 17;29(4):761-9. doi: 10.1038/emboj.2009.396. Epub 2010 Jan 14.
10
Unexpected diversity of RNase P, an ancient tRNA processing enzyme: challenges and prospects.核糖核酸酶P的意外多样性：古老的tRNA加工酶面临的挑战与前景
FEBS Lett. 2010 Jan 21;584(2):287-96. doi: 10.1016/j.febslet.2009.11.048.
本文引用的文献
1
Structural biology of Rad50 ATPase: ATP-driven conformational control in DNA double-strand break repair and the ABC-ATPase superfamily.Rad50 腺苷三磷酸酶的结构生物学：DNA 双链断裂修复中由三磷酸腺苷驱动的构象控制与 ABC 三磷酸腺苷酶超家族
Cell. 2000 Jun 23;101(7):789-800. doi: 10.1016/s0092-8674(00)80890-9.
2
Are DEAD-box proteins becoming respectable helicases?DEAD盒蛋白会成为受认可的解旋酶吗？
Nat Struct Biol. 2000 Feb;7(2):97-9. doi: 10.1038/72464.
3
Hera from Thermus thermophilus: the first thermostable DEAD-box helicase with an RNase P protein motif.嗜热栖热菌中的赫拉：首个具有核糖核酸酶P蛋白基序的热稳定DEAD盒解旋酶。
J Mol Biol. 1999 Dec 3;294(3):795-805. doi: 10.1006/jmbi.1999.3282.
4
ABC-ATPases, adaptable energy generators fuelling transmembrane movement of a variety of molecules in organisms from bacteria to humans.ABC转运ATP酶，是适应性的能量产生器，为从细菌到人类等各种生物体中多种分子的跨膜运动提供能量。
J Mol Biol. 1999 Oct 22;293(2):381-99. doi: 10.1006/jmbi.1999.2993.
5
Verification of phylogenetic predictions in vivo and the importance of the tetraloop motif in a catalytic RNA.体内系统发育预测的验证以及催化RNA中四环基序的重要性。
Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11200-5. doi: 10.1073/pnas.96.20.11200.
6
Localization in the nucleolus and coiled bodies of protein subunits of the ribonucleoprotein ribonuclease P.核糖核酸酶P核糖核蛋白的蛋白质亚基在核仁及卷曲小体中的定位
J Cell Biol. 1999 Aug 9;146(3):559-72. doi: 10.1083/jcb.146.3.559.
7
Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families.酿酒酵母中的RNA解旋：DEAD-box蛋白及相关家族
Trends Biochem Sci. 1999 May;24(5):192-8. doi: 10.1016/s0968-0004(99)01376-6.
8
Rpp14 and Rpp29, two protein subunits of human ribonuclease P.Rpp14和Rpp29，人核糖核酸酶P的两个蛋白质亚基。
RNA. 1999 Feb;5(2):153-7. doi: 10.1017/s135583829800185x.
9
Crystal structure of the ATP-binding subunit of an ABC transporter.一种ABC转运蛋白的ATP结合亚基的晶体结构。
Nature. 1998 Dec 17;396(6712):703-7. doi: 10.1038/25393.
Zotero 插件