完整的角膜上皮对于预防准分子原位角膜磨镶术后的基质 haze 至关重要。
Intact corneal epithelium is essential for the prevention of stromal haze after laser assisted in situ keratomileusis.
作者信息
Nakamura K, Kurosaka D, Bissen-Miyajima H, Tsubota K
机构信息
Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
出版信息
Br J Ophthalmol. 2001 Feb;85(2):209-13. doi: 10.1136/bjo.85.2.209.
Abstract
AIMS
To determine the effect of intact corneal epithelium on stromal haze and myofibroblast cell formation after excimer laser surgery.
METHODS
Denuded epithelium alone, photorefractive keratectomy (PRK), laser in situ keratomileusis (LASIK), or LASIK with denuded epithelium was performed in rabbit eyes. Postoperative anterior stromal haze was assessed employing a standard scale. Immunohistochemical methods were used to detect alpha smooth muscle actin (alpha-SMA), a marker for myofibroblastic cells, and type III collagen in subepithelial corneal tissue.
RESULTS
Three weeks after surgery, the presence of alpha-SMA positive long extended and spindle-shaped stromal cells, and synthesis of type III collagen were observed in the subepithelial stromal layer corresponding to corneal haze in PRK and LASIK with denuded epithelium, but not in denuded epithelium alone and LASIK.
CONCLUSION
The intact corneal epithelium may play an important part curbing subepithelial haze and differentiation of myofibroblasts in corneal wound healing.
摘要
目的
确定准分子激光手术后完整角膜上皮对基质混浊和肌成纤维细胞形成的影响。
方法
在兔眼中进行单纯上皮剥脱、准分子激光角膜切削术（PRK）、准分子激光原位角膜磨镶术（LASIK）或上皮剥脱的LASIK手术。采用标准量表评估术后前基质混浊情况。免疫组织化学方法用于检测角膜上皮下组织中α平滑肌肌动蛋白（α-SMA，肌成纤维细胞的标志物）和III型胶原。
结果
术后三周，在PRK和上皮剥脱的LASIK中，与角膜混浊相对应的上皮下基质层中观察到α-SMA阳性的长梭形基质细胞以及III型胶原的合成，而在单纯上皮剥脱和LASIK中未观察到。
结论
完整的角膜上皮可能在抑制角膜伤口愈合过程中的上皮下混浊和肌成纤维细胞分化方面发挥重要作用。
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本文引用的文献
1
Excimer laser photorefractive keratectomy for high myopia and myopic astigmatism.准分子激光屈光性角膜切削术治疗高度近视和近视散光。
Ophthalmic Surg Lasers. 1999 Jun;30(6):442-8.
2
Comparison of wound healing after photorefractive keratectomy and laser in situ keratomileusis in rabbits.兔眼准分子激光角膜切削术与准分子激光原位角膜磨镶术后伤口愈合情况的比较。
J Cataract Refract Surg. 1999 Jun;25(6):842-50. doi: 10.1016/s0886-3350(99)00047-4.
3
Extracellular matrix and growth factors in corneal wound healing.角膜伤口愈合中的细胞外基质与生长因子
Curr Opin Ophthalmol. 1995 Aug;6(4):3-10. doi: 10.1097/00055735-199508000-00002.
4
Photorefractive keratectomy for -1.25 to -25.00 diopters of myopia.用于治疗-1.25至-25.00屈光度近视的准分子激光原位角膜磨镶术。
J Refract Surg. 1998 Nov-Dec;14(6):615-22. doi: 10.3928/1081-597X-19981101-08.
5
SPARC from corneal epithelial cells modulates collagen contraction by keratocytes.角膜上皮细胞中的SPARC可调节角膜细胞的胶原收缩。
Invest Ophthalmol Vis Sci. 1998 Dec;39(13):2547-53.
6
Effects of transforming growth factor beta on corneal epithelial and stromal cell function in a rat wound healing model after excimer laser keratectomy.转化生长因子β对大鼠准分子激光角膜切削术后伤口愈合模型中角膜上皮和基质细胞功能的影响。
Graefes Arch Clin Exp Ophthalmol. 1998 Nov;236(11):834-43. doi: 10.1007/s004170050168.
7
Laser vision correction for low hyperopia. An 18-month assessment of safety and efficacy.低远视的激光视力矫正。安全性和有效性的18个月评估。
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8
9
Transforming growth factor-beta 1 promotes contraction of collagen gel by bovine corneal fibroblasts through differentiation of myofibroblasts.转化生长因子-β1通过肌成纤维细胞的分化促进牛角膜成纤维细胞对胶原凝胶的收缩。
Invest Ophthalmol Vis Sci. 1998 Apr;39(5):699-704.
10
Keratocyte apoptosis: implications on corneal wound healing, tissue organization, and disease.角膜细胞凋亡：对角膜伤口愈合、组织结构及疾病的影响
Invest Ophthalmol Vis Sci. 1998 Feb;39(2):220-6.
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