Nakamura K, Kurosaka D, Bissen-Miyajima H, Tsubota K
Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
Br J Ophthalmol. 2001 Feb;85(2):209-13. doi: 10.1136/bjo.85.2.209.
To determine the effect of intact corneal epithelium on stromal haze and myofibroblast cell formation after excimer laser surgery.
Denuded epithelium alone, photorefractive keratectomy (PRK), laser in situ keratomileusis (LASIK), or LASIK with denuded epithelium was performed in rabbit eyes. Postoperative anterior stromal haze was assessed employing a standard scale. Immunohistochemical methods were used to detect alpha smooth muscle actin (alpha-SMA), a marker for myofibroblastic cells, and type III collagen in subepithelial corneal tissue.
Three weeks after surgery, the presence of alpha-SMA positive long extended and spindle-shaped stromal cells, and synthesis of type III collagen were observed in the subepithelial stromal layer corresponding to corneal haze in PRK and LASIK with denuded epithelium, but not in denuded epithelium alone and LASIK.
The intact corneal epithelium may play an important part curbing subepithelial haze and differentiation of myofibroblasts in corneal wound healing.
确定准分子激光手术后完整角膜上皮对基质混浊和肌成纤维细胞形成的影响。
在兔眼中进行单纯上皮剥脱、准分子激光角膜切削术（PRK）、准分子激光原位角膜磨镶术（LASIK）或上皮剥脱的LASIK手术。采用标准量表评估术后前基质混浊情况。免疫组织化学方法用于检测角膜上皮下组织中α平滑肌肌动蛋白（α-SMA，肌成纤维细胞的标志物）和III型胶原。
术后三周，在PRK和上皮剥脱的LASIK中，与角膜混浊相对应的上皮下基质层中观察到α-SMA阳性的长梭形基质细胞以及III型胶原的合成，而在单纯上皮剥脱和LASIK中未观察到。
完整的角膜上皮可能在抑制角膜伤口愈合过程中的上皮下混浊和肌成纤维细胞分化方面发挥重要作用。
