Jaccoud D, Peng K, Feinstein D, Kilian A
Center for the Application of the Molecular Biology to International Agriculture (CAMBIA) Canberra, ACT, Australia.
Nucleic Acids Res. 2001 Feb 15;29(4):E25. doi: 10.1093/nar/29.4.e25.
Here we present the successful application of the microarray technology platform to the analysis of DNA polymorphisms. Using the rice genome as a model, we demonstrate the potential of a high-throughput genome analysis method called Diversity Array Technology, DArT'. In the format presented here the technology is assaying for the presence (or amount) of a specific DNA fragment in a representation derived from the total genomic DNA of an organism or a population of organisms. Two different approaches are presented: the first involves contrasting two representations on a single array while the second involves contrasting a representation with a reference DNA fragment common to all elements of the array. The Diversity Panels created using this method allow genetic fingerprinting of any organism or group of organisms belonging to the gene pool from which the panel was developed. Diversity Arrays enable rapid and economical application of a highly parallel, solid-state genotyping technology to any genome or complex genomic mixtures.
在此，我们展示了微阵列技术平台在DNA多态性分析中的成功应用。以水稻基因组为模型，我们证明了一种名为多样性阵列技术（DArT）的高通量基因组分析方法的潜力。在此呈现的形式中，该技术用于检测源自生物体或生物群体总基因组DNA的代表性样本中特定DNA片段的存在（或数量）。本文介绍了两种不同的方法：第一种方法是在单个阵列上对比两种代表性样本，而第二种方法是将一种代表性样本与阵列所有元件共有的参考DNA片段进行对比。使用这种方法创建的多样性面板可对属于该面板所源自的基因库的任何生物体或生物体群体进行基因指纹识别。多样性阵列能够将高度并行的固态基因分型技术快速且经济地应用于任何基因组或复杂的基因组混合物。
