Oder Gizem, Yuceer Semiha, Can Canan, Tanyolac Muhammed Bahattin, Ates Duygu
Department of Bioengineering, Ege University, Izmir, Turkey.
Phytopathology Department, Biological Control Research Institute, Adana, Turkey.
Sci Rep. 2025 Mar 6;15(1):7794. doi: 10.1038/s41598-025-87798-8.
Maize (Zea mays L.) is a frequently used food source in human and animal nutrition. Macrophomina phaseolina is a fungal pathogen causing charcoal rot disease in many plants, especially maize. This pathogen causes high yield losses in maize. The development of resistant maize genotypes is of great importance in controlling this disease. In this study, the population structure of 120 different maize genotypes with varying levels of disease resistance was determined and genome-wide association studies were performed. Each genotype was subjected to the pathogen under controlled conditions and their phenotypic responses to the disease were analyzed. Afterwards, single nucleotide polymorphisms were determined by DArT-seq sequencing. After filtering the SNP data, 37,470 clean SNPs were obtained. The population structure was analyzed with STRUCTURE software, and it was determined that the population was divided into two subgroups. The relationship between phenotypic and genotypic data was analyzed using the MLM (Q + K) model in TASSEL software. As a result, seven SNPs markers located on four different chromosomes were associated with disease resistance. The related markers can be used in the future for the development of maize varieties resistant to M. phaseolina by marker-assisted selection.
玉米（Zea mays L.）是人类和动物营养中常用的食物来源。菜豆壳球孢是一种真菌病原体，可在许多植物尤其是玉米中引发炭腐病。这种病原体导致玉米产量大幅损失。培育抗病玉米基因型对于控制这种病害至关重要。在本研究中，测定了120种不同抗病水平的玉米基因型的群体结构，并进行了全基因组关联研究。在可控条件下让每种基因型接触病原体，并分析它们对该病害的表型反应。之后，通过DArT-seq测序确定单核苷酸多态性。对SNP数据进行过滤后，获得了37470个纯净SNP。使用STRUCTURE软件分析群体结构，确定该群体分为两个亚组。在TASSEL软件中使用MLM（Q + K）模型分析表型和基因型数据之间的关系。结果，位于四条不同染色体上的七个SNP标记与抗病性相关。相关标记未来可用于通过标记辅助选择培育抗菜豆壳球孢的玉米品种。
