Gerold P, Schwarz R T
Med. Zentrum für Hygiene und Med. Mikrobiologie, Philipps-Universität, Robert-Koch-Strasse 17, 35037 Marburg, Germany.
Mol Biochem Parasitol. 2001 Jan 15;112(1):29-37. doi: 10.1016/s0166-6851(00)00336-4.
Glycolipids are important components of cellular membranes involved in various biological functions. In this report we describe the identification of the de-novo synthesis of glycosphingolipids by intraerythrocytic, asexual stages of the malaria parasite, Plasmodium falciparum. Parasite-specific glycolipids were identified in organic solvent extracts of parasites metabolically labeled with tritiated serine and glucosamine and characterised as sphingolipids based on their insensitivity towards alkaline treatment. While the de-novo synthesis of parasite glycosphingolipids was affected by fumonisin B1, threo-PPMP, cyclo-serine and myriocin, these well established inhibitors of de-novo ceramide biosynthesis were unable to arrest the intraerythrocytic development of the parasites in culture.
糖脂是参与各种生物学功能的细胞膜的重要组成部分。在本报告中，我们描述了疟原虫恶性疟原虫红细胞内无性阶段从头合成鞘糖脂的鉴定。在用氚标记的丝氨酸和葡糖胺进行代谢标记的寄生虫的有机溶剂提取物中鉴定出了寄生虫特异性糖脂，并根据它们对碱性处理的不敏感性将其表征为鞘脂。虽然寄生虫糖脂的从头合成受到伏马菌素B1、苏式-PPMP、环丝氨酸和米里霉素的影响，但这些已确立的从头神经酰胺生物合成抑制剂无法阻止培养中寄生虫的红细胞内发育。
