Zhu H, Snyder M
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
Curr Opin Chem Biol. 2001 Feb;5(1):40-5. doi: 10.1016/s1367-5931(00)00170-8.
In the past, studies of protein activities have focused on studying a single protein at a time, which is often time-consuming and expensive. Recently, with the sequencing of entire genomes, large-scale proteome analysis has begun. Arrays of proteins have been used for the determination of subcellular localization, analysis of protein-protein interactions and biochemical analysis of protein function. New protein-microarray technologies have been introduced that enable the high-throughput analysis of protein activities. These have the potential to revolutionize the analysis of entire proteomes.
过去，对蛋白质活性的研究一次只聚焦于研究一种蛋白质，这往往既耗时又昂贵。最近，随着全基因组测序的开展，大规模蛋白质组分析已经起步。蛋白质阵列已被用于亚细胞定位的测定、蛋白质 - 蛋白质相互作用的分析以及蛋白质功能的生化分析。新的蛋白质微阵列技术已经问世，能够对蛋白质活性进行高通量分析。这些技术有可能彻底改变对整个蛋白质组的分析。
