Saadatzadeh M R, Ashbee H R, Cunliffe W J, Ingham E
Division of Microbiology, Skin Research Centre, University of Leeds, Leeds LS2 9JT, UK.
Br J Dermatol. 2001 Jan;144(1):77-84. doi: 10.1046/j.1365-2133.2001.03955.x.
Malassezia is the aetiological agent of pityriasis versicolor. The mycelial phase of the organism predominates in lesions of pityriasis versicolor.
To evaluate the cell-mediated immune (CMI) response to the mycelial phase of Malassezia in patients with this disease, which has not previously been studied.
The CMI status of 12 patients with pityriasis versicolor and 12 age- and sex-matched controls to mycelial antigen(s) of the organism was examined. The responses to the mycelial form of three strains of the organism were assessed using lymphocyte transformation and leucocyte migration inhibition assays.
The transformation responses of the lymphocytes from both patients and controls gave transformation indices < or = 3, although the responses of lymphocytes from patients with pityriasis versicolor to the mycelial form of Malassezia strains were generally higher than those of the controls. In the leucocyte migration inhibition assay, leucocytes from patients with pityriasis versicolor and controls responded to the mycelial antigens of three different Malassezia strains; however, there was no significant difference in leucocyte response between patients with pityriasis versicolor and controls.
Patients with pityriasis versicolor do not therefore have a CMI deficiency to Malassezia mycelial antigens but fail to generate a protective CMI response to mycelial antigens over and above that of control individuals during active disease.
马拉色菌是花斑糠疹的病原体。该生物体的菌丝相在花斑糠疹皮损中占主导。
评估患有此病的患者对马拉色菌菌丝相的细胞介导免疫（CMI）反应，此前尚未对此进行过研究。
检测了12例花斑糠疹患者和12例年龄及性别匹配的对照对该生物体菌丝抗原的CMI状态。使用淋巴细胞转化试验和白细胞迁移抑制试验评估了对该生物体三株菌株菌丝形式的反应。
患者和对照的淋巴细胞转化反应的转化指数均≤3，尽管花斑糠疹患者的淋巴细胞对马拉色菌菌株菌丝形式的反应通常高于对照。在白细胞迁移抑制试验中，花斑糠疹患者和对照的白细胞对三种不同马拉色菌菌株的菌丝抗原均有反应；然而，花斑糠疹患者和对照之间的白细胞反应无显著差异。
因此，花斑糠疹患者对马拉色菌菌丝抗原不存在CMI缺陷，但在疾病活动期，除了对照个体外，未能对菌丝抗原产生保护性CMI反应。
