Peachman K K, Lyles D S, Bass D A
Department of Microbiology and Immunology, Wake Forrest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157-1054, USA.
Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1717-22. doi: 10.1073/pnas.98.4.1717.
In most eukaryotic cells, mitochondria use the respiratory chain to produce a proton gradient, which is then harnessed for the synthesis of ATP. Recently, mitochondrial roles in regulation of apoptosis have been discovered in many cell types. Eosinophils (Eos) die by apoptosis, but the presence and function of mitochondria in Eos are unknown. This study found that Eos contain mitochondria in small numbers, as shown by labeling with membrane potential-sensitive dyes and in situ PCR for a mitochondrial gene. Eos generate mitochondrial membrane potential from hydrolysis of ATP rather than from respiration, as shown by mitochondrial respiratory inhibitors and mitochondrial uncouplers. The mitochondria provide insignificant respiration but can induce apoptosis, as shown by using the mitochondrial F(1)F(0)-ATPase inhibitor oligomycin and translocation of cytochrome c. Thus during differentiation of Eos, although respiration is lost, the other central role of mitochondria, the induction of apoptosis, is retained.
在大多数真核细胞中，线粒体利用呼吸链产生质子梯度，然后利用该梯度合成ATP。最近，在许多细胞类型中发现了线粒体在细胞凋亡调节中的作用。嗜酸性粒细胞（Eos）通过凋亡死亡，但Eos中线粒体的存在和功能尚不清楚。本研究发现，用膜电位敏感染料标记和线粒体基因原位PCR显示，Eos含有少量线粒体。如线粒体呼吸抑制剂和线粒体解偶联剂所示，Eos通过ATP水解而非呼吸产生线粒体膜电位。线粒体提供的呼吸作用微不足道，但可诱导细胞凋亡，如使用线粒体F(1)F(0)-ATP酶抑制剂寡霉素和细胞色素c易位所示。因此，在Eos分化过程中，尽管呼吸作用丧失，但线粒体的另一个核心作用，即诱导细胞凋亡，得以保留。
