Wu Z, Dong G, Wan L
School of Stomatology, Fourth Military Medical University, Xi'an 710032.
Zhonghua Kou Qiang Yi Xue Za Zhi. 1997 Sep;32(5):300-2.
To evaluate biological effects in vitro of transforming growth factor-beta (TGF-beta) and/or insulin on proliferation of the human periodontal ligament (PDL) cells.
Cell cultures and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay.
TGF-beta at concentrations of 0.1 to 10 micrograms/L and insulin at 10 to 1,000 U/L had significant proliferative effects on human PDL cells (P < 0.01). From the 3rd day, the significant proliferation of PDL cells by TGF-beta or insulin were observed (P < 0.01) and lasted to the 5th day. The effect of combination of TGF-beta with insulin was the most significant one (P < 0.01).
TGF-beta and/or insulin may be valuable in promoting the regeneration of periodontal connective tissue.
评估转化生长因子-β（TGF-β）和/或胰岛素对人牙周膜（PDL）细胞增殖的体外生物学效应。
细胞培养和MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑]比色法。
浓度为0.1至10微克/升的TGF-β和10至1000单位/升的胰岛素对人PDL细胞具有显著的增殖作用（P < 0.01）。从第3天开始，观察到TGF-β或胰岛素对PDL细胞有显著增殖（P < 0.01），并持续到第5天。TGF-β与胰岛素联合使用的效果最为显著（P < 0.01）。
TGF-β和/或胰岛素在促进牙周结缔组织再生方面可能具有重要价值。
