Snijders A M, Meijer G A, Brakenhoff R H, van den Brule A J, van Diest P J
Department of Pathology, University Hospital Vrije Universiteit, Box 7057, 1007 MB Amsterdam, The Netherlands.
Mol Pathol. 2000 Dec;53(6):289-94. doi: 10.1136/mp.53.6.289.
Microarray technology allows the simultaneous analysis of up to thousands of different genes in histological or cytological specimens. Although microarray technology has so far mainly been applied in the research setting, its clinical application in pathology is expected in the foreseeable future. This paper presents an overview of the technical "ins and outs" of microarray technology, and discusses several putative applications in diagnostic pathology, which include tumour classification, the prediction of responses to certain chemotherapeutical or hormonal agents, the biological staging of tumours, the risk assessment of premalignant lesions, and the detection of microorganisms.
微阵列技术能够同时分析组织学或细胞学标本中多达数千种不同的基因。尽管迄今为止微阵列技术主要应用于研究领域,但在可预见的未来有望在病理学临床中得到应用。本文概述了微阵列技术的技术细节,并讨论了其在诊断病理学中的几种可能应用,包括肿瘤分类、对某些化疗药物或激素药物反应的预测、肿瘤的生物学分期、癌前病变的风险评估以及微生物检测。