Sanders C C, Sanders W E
Am J Clin Pathol. 1975 Mar;63(3):438-45. doi: 10.1093/ajcp/63.3.438.
Am J Clin Pathol 63:438-445, 1975. The effects of procedural variations on the activities of gentamicin, tobramycin, sisomicin, kanamycin, and amikacin in vitro were evaluated using 134 clinical isolates. In broth dilution studies, a change in assay medium from brain-heart infusion broth to Mueller-Hinton broth resulted in significant changes in minimal inhibitory concentrations in 36% (242 of 670) of assays. A change in the bacterial population size utilized in broth dilution studies resulted in significant changes in minimal inhibitory concentrations in 34% (155 of 456) of assays. These variations in activities appeared to depend more on the organism tested than on the particular aminoglycoside used; with strains of Staphylococcus aureus, Proteus, and Providenica being most affected. For all five aminoglycosides, minimal inhibitory concentrations determined by broth dilution, regardless of medium, showed poor correlation with zone sizes obtained by the Bauer-Kirby technic. These results suggest that unless some standard assay procedure for activity of aminoglycosides is adopted, meaningful comparison of results within and among laboratories will not be possible.
《美国临床病理学杂志》63:438 - 445, 1975年。使用134株临床分离菌评估了操作变化对庆大霉素、妥布霉素、西索米星、卡那霉素和阿米卡星体外活性的影响。在肉汤稀释研究中，测定培养基从脑心浸液肉汤改为穆勒-欣顿肉汤导致36%（670次测定中的242次）的最小抑菌浓度出现显著变化。肉汤稀释研究中使用的细菌群体大小的变化导致34%（456次测定中的155次）的最小抑菌浓度出现显著变化。这些活性变化似乎更多地取决于所测试的生物体，而非所使用的特定氨基糖苷类药物；金黄色葡萄球菌、变形杆菌和普罗威登斯菌菌株受影响最大。对于所有五种氨基糖苷类药物，无论培养基如何，通过肉汤稀释测定的最小抑菌浓度与通过鲍尔-柯比技术获得的抑菌圈大小相关性较差。这些结果表明，除非采用某种氨基糖苷类药物活性的标准测定程序，否则各实验室内部及之间的结果进行有意义的比较将是不可能的。
