Tawfeek G M, Hussein D A
Department of Parasitology and Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
J Egypt Soc Parasitol. 2000 Dec;30(3):679-97.
This study included 71 individuals: 25 patients with confirmed acute fascioliasis, 9 patients with suspected fascioliasis, 27 patients with parasitic infections other than fascioliasis and 10 normal controls. Patients with fascioliasis were treated with TCZ as a single dose and followed up 1 and 4 months after therapy. The results showed that both IgM and IgG cystatin capture ELISAs had very high sensitivity and specificity of 100%. They were also able to diagnose early fascioliasis even before indirect haemagglutination test (IHAT) became positive. Within 4 months after treatment, only 20 patients (80%) with fascioliasis were cured as evidenced clinically, by normal eosinophil count and negative IHAT. After the first month of treatment, IgM and IgG cystatin capture ELISAs became negative in 16.6% and 83.3% of the cured cases respectively, reaching 95% for both assays after 4 months. They remained positive in 5 cases (20%) not responding to treatment.
本研究纳入了71名个体：25例确诊为急性片形吸虫病的患者、9例疑似片形吸虫病的患者、27例患有除片形吸虫病之外的寄生虫感染的患者以及10名正常对照。片形吸虫病患者接受单剂量的三氮脒治疗，并在治疗后1个月和4个月进行随访。结果显示，IgM和IgG胱抑素捕获ELISA的敏感性和特异性均非常高，均为100%。它们甚至能够在间接血凝试验（IHAT）呈阳性之前诊断早期片形吸虫病。治疗后4个月内，仅20例（80%）片形吸虫病患者临床治愈，嗜酸性粒细胞计数正常且IHAT呈阴性。治疗第一个月后，IgM和IgG胱抑素捕获ELISA在分别16.6%和83.3%的治愈病例中转为阴性，4个月后两种检测均达到95%。在5例（20%）治疗无反应的病例中，它们仍为阳性。
