Kaplan A E, Picca M, Gonzalez M I, Macchi R L, Molgatini S L
Department of Dental Materials, School of Dentistry, University of Buenos Aires, Argentina.
Endod Dent Traumatol. 1999 Feb;15(1):42-5. doi: 10.1111/j.1600-9657.1999.tb00748.x.
The aim of this study was to determine the in vitro antimicrobial effect of six endodontic sealers after 2, 20 and 40 days. The sealers studied were Apexit, Endion, AH26, AH-Plus. Procosol and Ketac Endo. The microorganisms used were Candida albicans, Staphylococcus aureus, Streptococcus mutans and Actinomyces israelii. Petri dishes were filled with sterile agar and 0.1-ml wells were prepared and filled with the sealers. The agar plates were stored for 24 h at 37 degrees C. The samples were then removed, immersed in 4.5 ml of culture medium and divided into three groups. The samples in group 1 were stored for 2 days at 37 degrees C whereas the samples of groups 2 and 3 were stored at 4 degrees C for 20 and 40 days respectively. The samples were then removed and discarded, and 0.1 ml of the culture medium was seeded on the agar plates in order to perform colony forming unit counts. Apexit, Endion and AH-Plus produced slight inhibition on Streptococcus mutans at 20 days and on Actinomyces israelii at every time interval. No effect was found on Candida albicans and Staphylococcus aureus. Ketac Endo only produced an antimicrobial effect on Actinomyces israelii at 2 and 40 days. AH26 and Procosol showed antimicrobial effect at 40 days on Candida albicans, at 20 and 40 days on Streptococcus mutans and Staphylococcus aureus, and an effective inhibition on Actinomyces israelii at every time interval. Statistical analysis revealed both sealers and microorganisms to be significant factors affecting results in groups 2 and 3. In conclusion, the sealers evaluated in this study showed different inhibitory effects depending on time span. Overall, sealers containing cugenol and formaldehyde proved to be most effective against the microorganisms at the time intervals studied.
本研究的目的是确定六种根管封闭剂在2天、20天和40天后的体外抗菌效果。所研究的封闭剂有Apexit、Endion、AH26、AH-Plus、Procosol和Ketac Endo。所使用的微生物有白色念珠菌、金黄色葡萄球菌、变形链球菌和衣氏放线菌。将培养皿装满无菌琼脂，制备0.1毫升的孔并填入封闭剂。琼脂平板在37℃下保存24小时。然后取出样品，浸入4.5毫升培养基中并分成三组。第1组的样品在37℃下保存2天，而第2组和第3组的样品分别在4℃下保存20天和40天。然后取出并丢弃样品，将0.1毫升培养基接种在琼脂平板上以进行菌落形成单位计数。Apexit、Endion和AH-Plus在20天时对变形链球菌有轻微抑制作用，在每个时间间隔对衣氏放线菌都有抑制作用。对白色念珠菌和金黄色葡萄球菌没有作用。Ketac Endo仅在2天和40天时对衣氏放线菌产生抗菌作用。AH26和Procosol在40天时对白色念珠菌有抗菌作用，在20天和40天时对变形链球菌和金黄色葡萄球菌有抗菌作用，并且在每个时间间隔对衣氏放线菌都有有效抑制作用。统计分析表明，封闭剂和微生物都是影响第2组和第3组结果的重要因素。总之，本研究中评估的封闭剂根据时间跨度显示出不同的抑制作用。总体而言，在所研究的时间间隔内，含丁香酚和甲醛的封闭剂对微生物最有效。
