Stokes G B, Tove S B
J Biol Chem. 1975 Aug 25;250(16):6315-9.
The proportion of palmitate at position 2 of triacylglycerols synthesized by microsome-cytosol preparations of rat, mouse, or chicken adipose tissue was found to be markedly increased by the addition of an inactivated preparation of pig adipose tissue microsomes. A phosphate extract of pig adipose tissue microsomes was also active in directing palmitate to position 2 of rat triacylglycerols. The proportion of palmitate at position 2 of triacylglycerols synthesized was reduced by prior repeated extraction of the pig adipose tissue microsomes with phosphate. The pretreatment specificity was restored when the extract was added back to the microsomes. These observations support the proposal that the unusually high proportion of palmitate at position 2 of lard results from the action of a factor controlling the specificity of the acyltransferase(s) from pig adipose tissue.
研究发现，添加经灭活处理的猪脂肪组织微粒体后，大鼠、小鼠或鸡脂肪组织的微粒体 - 胞质溶胶制剂合成的三酰甘油中，2位棕榈酸酯的比例显著增加。猪脂肪组织微粒体的磷酸盐提取物在引导棕榈酸酯至大鼠三酰甘油的2位时也具有活性。预先用磷酸盐反复提取猪脂肪组织微粒体后，合成的三酰甘油中2位棕榈酸酯的比例降低。当将提取物重新添加回微粒体时，预处理特异性得以恢复。这些观察结果支持以下观点：猪油中2位棕榈酸酯比例异常高是由于一种控制猪脂肪组织酰基转移酶特异性的因子作用所致。
