成年大鼠肾脏中9-羟基前列腺素脱氢酶的活性。区域分布与亚分级分离

9-Hydroxyprostaglandin dehydrogenase activity in the adult rat kidney. Regional distribution and sub-fractionation.

Asciak C P, Domazet Z

Biochim Biophys Acta. 1975 Feb 20;380(2):338-43. doi: 10.1016/0005-2760(75)90019-3.

Abstract

Catabolism of prostaglandin F2alpha in the adult rat kidney takes place by the following sequence of enzymatic steps: (1) 15-hydroxyprostaglandin dehydrogenase; (2) prostaglandin delta13-reductase; and (3) 9-hydroxyprostaglandin dehydrogenase. 2. 9-Hydroxyprostaglandin dehydrogenase activity was highest in the cortex with lesser amounts in the medulla and negligible activity detected in the papilla. A similar distribution was observed for 15-hydroxyprostaglandin dehydrogenase and prostaglandin delta13-reductase. 3. Most of the 9-hydroxyprostaglandin dehydrogenase activity in the homogenate was found in the high-speed supernatant as also observed for 15-hydroxyprostaglandin dehydrogenase and prostaglandin delta13-reductase. 4. These observations indicate that the rat kidney contains an abundance of prostaglandin-catabolising enzymes which favour formation of metabolites of the E-type.

摘要

成年大鼠肾脏中前列腺素F2α的分解代谢按以下酶促步骤顺序进行：(1) 15-羟基前列腺素脱氢酶；(2) 前列腺素δ13-还原酶；以及(3) 9-羟基前列腺素脱氢酶。2. 9-羟基前列腺素脱氢酶活性在皮质中最高，髓质中含量较少，乳头中检测到的活性可忽略不计。15-羟基前列腺素脱氢酶和前列腺素δ13-还原酶也观察到类似的分布。3. 匀浆中大部分9-羟基前列腺素脱氢酶活性存在于高速上清液中，15-羟基前列腺素脱氢酶和前列腺素δ13-还原酶也是如此。4. 这些观察结果表明，大鼠肾脏含有丰富的前列腺素分解代谢酶，有利于E型代谢物的形成。