Cui L, Fan Y
Research Institute of Highland and Cold Zone Crops, Academy of Agricultural Sciences of Shanxi, Datong, China.
Chin J Biotechnol. 1998;14(1):31-6.
The original calli were obtained by inducing culture of mature embryos of naked seed oat on N6 medium. The original calli were white-colored tumor forms, soft outside and hard inside. These kinds of calli are easy to differentiate into plantlets, and they are not the friable type. Friable embryogenic calli could be obtained by cycled regulated culture on IM1-IM4 medium for 7-8 months from the original calli. They became vigorous, lightish yellow in color, with small grainy forms. Well-separated and fast-growing suspending cell lines have been obtained from the above-mentioned embryogenic calli in the liquid medium. Regenerated plants have been obtained for this kind of suspension line by culturing on the medium for differentiation. The surviving percentage for such plantlets was over 95% after planting in the soil.
原始愈伤组织是通过将裸燕麦成熟胚在N6培养基上进行诱导培养获得的。原始愈伤组织呈白色瘤状，外软内硬。这类愈伤组织易于分化成植株，且不属于易碎型。通过在IM1 - IM4培养基上循环调控培养7 - 8个月，可从原始愈伤组织获得易碎胚性愈伤组织。它们生长旺盛，颜色呈浅黄色，形态为小颗粒状。在液体培养基中，已从上述胚性愈伤组织获得了分离良好且生长迅速的悬浮细胞系。通过在分化培养基上培养，已从这种悬浮系获得了再生植株。将此类植株移栽到土壤中后，其成活率超过95%。
