Literak I, Robesova B, Majlathova V, Majlath I, Kulich P, Fabian P, Roubalova E
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 612 42 Brno, Czech Republic.
J Wildl Dis. 2010 Jan;46(1):257-61. doi: 10.7589/0090-3558-46.1.257.
Papillomatous skin lesions from a green lizard (Lacerta viridis) were examined histologically, using electron microscopy and DNA was isolated from the lesions for herpes-viral DNA detection. Histology confirmed the lesions to be squamous epithelial papillomas. Using electron microscopy, no virus particles were detected. The specific sequence of herpesviral DNA-directed DNA polymerase (EC 2.7.7.7) was amplified using degenerate primers in a nested format. The 235-base-pair (bp) sequence was sequenced and compared with previously published DNA-directed DNA polymerase sequences from various reptile herpesviruses. The sequence from the green lizard showed significant similarity with sequence of fibropapilloma-associated turtle herpesviruses from sea turtles.
对一只绿蜥蜴（绿蜥蜴属）的乳头状皮肤病变进行了组织学检查，使用电子显微镜，并从病变中分离出DNA用于检测疱疹病毒DNA。组织学证实病变为鳞状上皮乳头状瘤。使用电子显微镜未检测到病毒颗粒。使用简并引物以巢式形式扩增疱疹病毒DNA指导的DNA聚合酶（EC 2.7.7.7）的特定序列。对235个碱基对（bp）的序列进行测序，并与先前发表的来自各种爬行动物疱疹病毒的DNA指导的DNA聚合酶序列进行比较。绿蜥蜴的序列与来自海龟的纤维乳头状瘤相关海龟疱疹病毒的序列显示出显著的相似性。
