Sufka K J, Roach J T, Chambliss W G, Broom S L, Feltenstein M W, Wyandt C M, Zeng L
Department of Psychology, University of Mississippi, Oxford 38677, USA.
Psychopharmacology (Berl). 2001 Jan 1;153(2):219-24. doi: 10.1007/s002130000571.
The recent growth in sales of natural products labeled as dietary supplements in the United States has renewed scientific interest in the study of the therapeutic effects of multi-component botanical products.
This study sought to determine whether botanical extracts derived from the Rutaceae family, Acori graminei, the Magnoliaceae family, Alchemilla vulgaris and Primula veris, which had previously been identified in bioassays as having potential anxiolytic activity, were active in the chick social separation-stress procedure.
Eight-day-old chicks received IP injections of test articles 30 min before being tested in the presence of two social companions or in isolation for a 3-min observation period. Dependent measures were: a) latency to adopt a ventral recumbent posture to index sedation, b) number of vocalizations to index separation-distress and c) a composite pain score (comprised of footlift frequency and footlift duration in response to 50 microl of 0.10% formalin injected into the plantar surface of the foot) to index stress-induced analgesia.
Proprietal extracts NPS00033 from the Rutaceae plant family and NPS00039 (Relora) from the Magnoliaceae plant family screened positive in this chick model without causing sedation.
These results suggest that botanical extracts Relora and NPS00033 may be useful in modulating anxiety states.
近期，美国标注为膳食补充剂的天然产品销量增长，这重新激发了科学界对多成分植物产品治疗效果研究的兴趣。
本研究旨在确定从芸香科、石菖蒲、木兰科、普通羽衣草和报春花中提取的植物提取物，这些提取物在生物测定中先前已被确定具有潜在的抗焦虑活性，在雏鸡社会隔离应激程序中是否具有活性。
8日龄雏鸡在有两个同伴在场或单独隔离的情况下接受3分钟观察期测试前30分钟腹腔注射受试物。相关测量指标为：a）采用腹卧位姿势的潜伏期以指示镇静程度，b）鸣叫次数以指示分离痛苦程度，c）综合疼痛评分（由对注入足底表面50微升0.10%福尔马林的抬脚频率和抬脚持续时间组成）以指示应激诱导的镇痛作用。
芸香科植物家族的Proprietal提取物NPS00033和木兰科植物家族的NPS00039（Relora）在该雏鸡模型中筛选呈阳性，且未引起镇静作用。
这些结果表明，植物提取物Relora和NPS00033可能有助于调节焦虑状态。
