Zurburg W, Ebels I
J Neural Transm. 1975;36(1):59-69. doi: 10.1007/BF01243438.
After separation of aqueous extracts of sheep pineal bodies and sheep cerebral cortex on Sephadex G-25, a high fluorescence peak showing an excitation maximum at 305 leads to 310 nm and a fluorescence maximum at 350 leads to 355 nm was observed in pineal extracts, which could not be detected in cerebral cortex extracts. Separation of this pineal fluorescence peak on Sephadix G-10 with water elution, followed by gradient elution of water and NaOH, gave rise to several highly fluorescent peaks. From one of these it was possible to isolate a substance with Rf-values, using thinlayer chromatographic, fluorescence and mass spectra studies, identical with synthetic 5-hydroxyindole-3-acetic acid. From another peak a substance, idential with synthetic 5-methoxyindole-3-acetic acid, could be isolated. The results are compared with those obtained in previous studies.
在对绵羊松果体和绵羊大脑皮层的水提取物进行葡聚糖凝胶G - 25柱层析分离后，在松果体提取物中观察到一个高荧光峰，其激发最大值在305至310纳米之间，荧光最大值在350至355纳米之间，而在大脑皮层提取物中未检测到该峰。用蒸馏水对该松果体荧光峰进行葡聚糖凝胶G - 10柱层析分离，随后用蒸馏水和氢氧化钠进行梯度洗脱，产生了几个高荧光峰。通过薄层色谱、荧光和质谱研究，从其中一个峰中分离出一种物质，其比移值与合成的5 - 羟基吲哚 - 3 - 乙酸相同。从另一个峰中可以分离出一种与合成的5 - 甲氧基吲哚 - 3 - 乙酸相同的物质。将这些结果与先前研究中获得的结果进行了比较。
