Maticic M, Poljak M, Kramar B, Seme K, Brinovec V, Meglic-Volkar J, Zakotnik B, Skaleric U
Department of Infectious Diseases, University Medical Centre, Ljubljana, Slovenia.
J Periodontol. 2001 Jan;72(1):11-6. doi: 10.1902/jop.2001.72.1.11.
To search for a possible source of hepatitis C virus (HCV) in saliva, the presence and shedding patterns of HCV in gingival crevicular fluid (GCF) and saliva of HCV viremic patients were assessed based on clinical, biochemical, histological, virological, and oral health parameters.
Saliva and GCF samples of 50 HCV viremic patients were collected to detect HCV RNA by a modified commercial polymerase chain reaction (PCR) assay. Clinical oral examination was performed and periodontal status at the collection sites was monitored. The results were correlated to specified parameters.
HCV RNA was detected in 59% (29/49) of the GCF specimens and in 35% (17/48) of the saliva specimens. In saliva specimens, HCV RNA was detected only in cases which also had detectable HCV RNA in the GCF samples (P=0.00002) and was significantly related to the presence of blood in saliva (P=0.03). Higher, but not significant, values of oral clinical parameters at the sites of fluid collection were found in GCF specimens harboring HCV RNA. In GCF specimens with no blood detected, HCV RNA was more often present in cases with higher plasma viral load (P=0.05).
The results suggest that besides blood, the other most probable source of HCV in saliva is GCF. Unknown endogenous HCV inhibitory mechanisms in the oral cavity may explain the discrepancies in HCV appearance between saliva and GCF. The results provide a biologic basis for further investigation of the role of HCV in the pathogenesis of periodontal disease.
为了寻找丙型肝炎病毒（HCV）在唾液中的可能来源，基于临床、生化、组织学、病毒学和口腔健康参数，评估了HCV病毒血症患者龈沟液（GCF）和唾液中HCV的存在情况及排出模式。
收集50例HCV病毒血症患者的唾液和GCF样本，通过改良的商业聚合酶链反应（PCR）检测法检测HCV RNA。进行临床口腔检查并监测采集部位的牙周状况。将结果与特定参数相关联。
在59%（29/49）的GCF样本和35%（17/48）的唾液样本中检测到HCV RNA。在唾液样本中，仅在GCF样本中也检测到可检测HCV RNA的病例中检测到HCV RNA（P=0.00002），并且与唾液中血液的存在显著相关（P=0.03）。在含有HCV RNA的GCF样本中，发现液体采集部位的口腔临床参数值较高，但无显著差异。在未检测到血液的GCF样本中，血浆病毒载量较高的病例中更常出现HCV RNA（P=0.05）。
结果表明，除血液外，唾液中HCV的另一个最可能来源是GCF。口腔中未知的内源性HCV抑制机制可能解释了唾液和GCF之间HCV出现的差异。这些结果为进一步研究HCV在牙周疾病发病机制中的作用提供了生物学基础。
