De Giglio E, Sabbatini L, Colucci S, Zambonin G
Dipartimento di Chimica, Universita' degli Studi di Bari, Italy.
J Biomater Sci Polym Ed. 2000;11(10):1073-83. doi: 10.1163/156856200743580.
The covalent attachment of an Arg-Gly-Asp (RGD) containing peptide to polypyrrole(PPy)-coated titanium substrates has been investigated in order to develop a bioactive material of potential use in orthopedic fields. Polypyrrole has been employed as the coating polymer because of its suitability to be electrochemically grown directly onto metallic substrates of different shapes, leading to remarkably adherent films. The synthetic peptide Cys-Gly-(Arg-Gly-Asp)-Ser-Pro-Lys, containing the cell-adhesive region of fibronectin (RGD), has been grafted to the polymer substrate via the cysteine residue using a procedure recently developed in the authors laboratory. The effectiveness of grafting was monitored by X-ray photoelectron spectroscopy (XPS), which assessed the presence of the peptide grafted onto the polymer surface exploiting the cysteine sulfur as target element. Neonatal rat calvarial osteoblasts were attached to RGD-modified PPy-coated Ti substrates at levels significantly greater than on unmodified PPy-coated Ti and glass coverslip substrates.
为了开发一种在骨科领域具有潜在用途的生物活性材料，人们研究了将含精氨酸 - 甘氨酸 - 天冬氨酸（RGD）的肽共价连接到聚吡咯（PPy）涂层钛基底上的方法。聚吡咯因其适合通过电化学方法直接生长在不同形状的金属基底上，从而形成附着力极强的薄膜，而被用作涂层聚合物。合成肽半胱氨酸 - 甘氨酸 -（精氨酸 - 甘氨酸 - 天冬氨酸）- 丝氨酸 - 脯氨酸 - 赖氨酸，包含纤连蛋白的细胞黏附区域（RGD），已通过作者实验室最近开发的一种方法，利用半胱氨酸残基接枝到聚合物基底上。通过X射线光电子能谱（XPS）监测接枝的有效性，该技术以半胱氨酸硫作为目标元素来评估接枝到聚合物表面的肽的存在情况。新生大鼠颅骨成骨细胞附着在RGD修饰的PPy涂层钛基底上的水平显著高于未修饰的PPy涂层钛基底和玻璃盖玻片基底。
