Shen Xuexue, Gill Upinder, Arens Marjon, Yan Zhe, Bai Yuling, Hutton Samuel F, Wolters Anne-Marie A
Plant Breeding, Wageningen University and Research, Wageningen, The Netherlands.
Graduate School Experimental Plant Sciences, Wageningen University and Research, Wageningen, The Netherlands.
Theor Appl Genet. 2025 Jan 8;138(1):22. doi: 10.1007/s00122-024-04803-w.
The tomato Ty-6 gene conferring resistance against begomoviruses has been cloned and shown to be a variant of DNA polymerase delta subunit 1. Ty-6 is a major resistance gene of tomato that provides resistance against monopartite and bipartite begomoviruses. The locus was previously mapped on chromosome 10, and in this study, we fine-mapped Ty-6 to a region of 47 kb, including four annotated candidate genes. Via whole-genome resequencing of Ty-6 breeding lines and several susceptible breeding lines, the polymorphisms in gene sequences were discovered and gene-associated markers were developed for marker-assistant breeding. Further, virus-induced gene silencing and candidate gene overexpressing in susceptible tomatoes revealed that Ty-6-mediated resistance is controlled by Solyc10g081250, encoding the DNA polymerase delta subunit 1, SlPOLD1. The single nucleotide polymorphism of Ty-6 results in an amino acid change that might influence the fidelity of virus DNA replication.
赋予番茄对双生病毒抗性的Ty-6基因已被克隆，并且被证明是DNA聚合酶δ亚基1的一个变体。Ty-6是番茄的一个主要抗性基因，可提供对单分体和双分体双生病毒的抗性。该基因座先前被定位在第10号染色体上，在本研究中，我们将Ty-6精细定位到一个47 kb的区域，其中包括四个注释的候选基因。通过对Ty-6育种系和几个感病育种系进行全基因组重测序，发现了基因序列中的多态性，并开发了与基因相关的标记用于标记辅助育种。此外，在感病番茄中进行病毒诱导的基因沉默和候选基因过表达分析表明，Ty-6介导的抗性受Solyc10g081250控制，该基因编码DNA聚合酶δ亚基1，即SlPOLD1。Ty-6的单核苷酸多态性导致氨基酸变化，这可能会影响病毒DNA复制的保真度。
