Başkurt O K, Mat F
Department of Physiology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Clin Hemorheol Microcirc. 2000;23(1):43-9.
It is well known that RBC rheological parameters are affected by temperature. Usually, the measurement temperature of these parameters is kept constant throughout a given study, however it can be seen that different temperatures are used by different groups, during the measurement of a given parameter. It is assumed that the data should not be qualitatively different when measured at different temperatures, although significant quantitative differences exist. This study revealed that the selected temperature for RBC elongation index measurements by ektacytometry is important in detecting a given impairment in RBC deformability induced by experimental sepsis. RBC elongation indexes were found to be significantly different in septic and normal rats, only if measured at 37 degrees C. The differences in RBC aggregation parameters for septic and normal rats were also affected by the measurement temperature, however statistically significant differences were present in a wider range of temperatures between 25-37 degrees C. Therefore, it is strongly recommended that the measurement of RBC deformability and aggregation be performed using a controlled-temperature device, set to 37 degrees C.
众所周知，红细胞流变学参数受温度影响。通常，在给定研究中这些参数的测量温度保持恒定，然而可以看出，在测量给定参数时，不同的研究小组使用不同的温度。尽管存在显著的定量差异，但假定在不同温度下测量时数据在定性上不应有差异。本研究表明，通过激光衍射血细胞分析仪测量红细胞伸长指数时，所选温度对于检测实验性脓毒症诱导的红细胞变形性的特定损伤很重要。仅在37℃测量时，才发现脓毒症大鼠和正常大鼠的红细胞伸长指数有显著差异。脓毒症大鼠和正常大鼠的红细胞聚集参数差异也受测量温度影响，然而在25 - 37℃的更宽温度范围内存在统计学显著差异。因此，强烈建议使用设置为37℃的控温装置进行红细胞变形性和聚集性的测量。
