Liu H, Liu J, Xiong S, Shen G, Zhang Z, Xu Y
Department of Internal Medicine, Tongji Hospital, Tongji Medical University, Wuhan 430030.
J Tongji Med Univ. 2000;20(3):200-2. doi: 10.1007/BF02886988.
The levels of lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) expression in culture of peripheral blood mononuclear cells (PBMC) and the plasma levels of IL-6 and TNF-alpha in the patients with obstructive sleep apnea syndrome (OSAS) were measured and the relationship between OSAS and IL-6 or TNF-alpha expression studied. Both IL-6 and TNF-alpha were detected by using ELISA in 22 patients with OSAS and 16 normal controls. The levels of LPS-induced IL-6 (787.82 +/- 151.97 pg/ml) and TNF-alpha (4165.45 +/- 1501.43 pg/ml) expression in the supernatant of the culture of PBMC and plasma level of IL-6 (50.67 +/- 4.70 pg/ml) and TNF-alpha (299.09 +/- 43.57 pg/ml) in the patients with OSAS were significantly higher than those in the normal controls (in the supernatant of the culture of PBMC: 562.69 +/- 197.54 pg/ml and 1596.25 +/- 403.08 pg/ml respectively; in the plasma; 12.69 +/- 2.75 pg/ml and 101.88 +/- 21.27 pg/ml respectively). There were significantly positive correlation between the levels of IL-6 and TNF-alpha and the percentage of time of apnea and hyponea, as well as the percentage of time spending at SaO2 below 90% in the total sleep time. It was concluded that LPS-induced IL-6 and TNF-alpha levels as well as plasma IL-6 and TNF-alpha levels in the patients with OSAS were up-regulated, which may be associated with the pathogenesis of OSAS.
检测阻塞性睡眠呼吸暂停综合征（OSAS）患者外周血单个核细胞（PBMC）培养物中脂多糖（LPS）诱导的白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）表达水平以及血浆中IL-6和TNF-α水平，并研究OSAS与IL-6或TNF-α表达之间的关系。采用酶联免疫吸附测定法（ELISA）检测22例OSAS患者和16例正常对照者的IL-6和TNF-α。OSAS患者PBMC培养上清液中LPS诱导的IL-6（787.82±151.97 pg/ml）和TNF-α（4165.45±1501.43 pg/ml）表达水平以及血浆中IL-6（50.67±4.70 pg/ml）和TNF-α（299.09±43.57 pg/ml）水平显著高于正常对照者（PBMC培养上清液中分别为562.69±197.54 pg/ml和1596.25±403.08 pg/ml；血浆中分别为12.69±2.75 pg/ml和101.88±21.27 pg/ml）。IL-6和TNF-α水平与呼吸暂停和低通气时间百分比以及总睡眠时间中血氧饱和度（SaO2）低于90%的时间百分比之间存在显著正相关。结论：OSAS患者LPS诱导的IL-6和TNF-α水平以及血浆IL-6和TNF-α水平上调，这可能与OSAS的发病机制有关。
