Kaur S, Grover I S, Kumar S
Department of Botanical Sciences, Guru Nanak Dev University, Amritsar, India.
J Environ Pathol Toxicol Oncol. 2001;20(1):9-14.
Terminalia arjuna is an important medicinal plants widely used in the preparation of Ayurvedic formulations used against several ailments. The present investigation was aimed at the fractionation of crude extracts from the bark of T. arjuna in order to isolate and purify the antimutagenic factors present. The antimutagenicity assay was performed to check the modulatory effect of these fractions against NPD, sodium azide, and 2AF, using the Ames Salmonella his+ reversion assay. Most of the phenolic fractions exhibited mutagen specificity against direct-acting mutagens, being effective in suppressing the frameshift mutagen NPD but failing to inhibit sodium azide (base pair substitution)-induced his+ revertants. ET-1 fraction triterpenoid diglycoside showed a marked effect against sodium azide but was ineffective against NPD. In the case of the indirect-acting mutagen 2AF, all the fractions were found to be quite potent in modulating its mutagenicity in both TA98 and TA100 tester strains of Salmonella typhimurium. The results indicate that the bark of T. arjuna harbors constituents with promising antimutagenic/anticarcinogenic potential that should be investigated further.
诃子是一种重要的药用植物，广泛用于制备阿育吠陀配方药物，用于治疗多种疾病。本研究旨在对诃子树皮的粗提物进行分离，以分离和纯化其中存在的抗诱变因子。采用鼠伤寒沙门氏菌组氨酸回复突变试验，进行抗诱变性试验，以检测这些组分对NPD、叠氮化钠和2AF的调节作用。大多数酚类组分对直接作用诱变剂表现出诱变特异性，能有效抑制移码诱变剂NPD，但不能抑制叠氮化钠（碱基对置换）诱导的组氨酸回复突变体。ET-1组分三萜双糖苷对叠氮化钠有显著作用，但对NPD无效。对于间接作用诱变剂2AF，发现所有组分在调节鼠伤寒沙门氏菌TA98和TA100测试菌株中的诱变性方面都相当有效。结果表明，诃子树皮含有具有潜在抗诱变/抗癌潜力的成分，值得进一步研究。
