Arora Saroj, Kaur Kamaljit, Kaur Swayamjot
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, India.
Teratog Carcinog Mutagen. 2003;Suppl 1:295-300. doi: 10.1002/tcm.10055.
India is one of the 12 mega diversity countries in the world so it has a vital stake in conservation and sustainable utilization of its biodiversity resources. Plant secondary metabolites have been of interest to man for a long time due to their pharmacological relevance. With this in view, the bark powder of Acacia auriculiformis, A. nilotica, Juglans regia, and the fruit powder of Terminalia bellerica, T. chebula, Emblica officinalis, and a combination drug "Triphala," which are known to be rich in polyphenols, were tested for their antimutagenic activities. Antimutagenic activities of the extracts were estimated by employing the plate incorporation Ames Salmonella histidine reversion assay by using the frame shift mutagen tester strain TA98 and base pair substitution strain TA100 against direct acting mutagens (NPD, sodium azide), and the S9-dependent mutagen 2-aminofluorene(2AF). Acetone extracts of all the plants exhibited significant antimutagenic activities among the other extracts tested, but an acetone extract of Acacia nilotica showed a marked anti-mutagent effect. Furthermore, it was more effective against indirect acting mutagen, 2AF, in both TA98 and TA100 tester strains of Salmonella typhimurium than against the direct acting mutagens. The results indicate that an acetone extract of bark and fruit of the medicinal plants under study harbors constituents with promising antimutagenic/anticarcinogenic potential that could be investigated further.
印度是世界上12个生物多样性大国之一，因此在保护和可持续利用其生物多样性资源方面有着至关重要的利害关系。由于植物次生代谢产物具有药理学相关性，长期以来一直受到人们的关注。鉴于此，对富含多酚的耳叶相思树、尼罗罗非鱼、胡桃的树皮粉，以及诃子、毗黎勒、余甘子的果粉和一种复方药物“三果木”进行了抗诱变活性测试。通过使用移码诱变测试菌株TA98和碱基对替代菌株TA100，采用平板掺入艾姆斯沙门氏菌组氨酸回复突变试验，针对直接作用诱变剂（NPD、叠氮化钠）和S9依赖性诱变剂2-氨基芴（2AF），评估提取物的抗诱变活性。在所测试的其他提取物中，所有植物的丙酮提取物均表现出显著的抗诱变活性，但尼罗罗非鱼的丙酮提取物显示出明显的抗突变作用。此外，在鼠伤寒沙门氏菌的TA98和TA100测试菌株中，它对间接作用诱变剂2AF的效果比对直接作用诱变剂的效果更有效。结果表明，所研究药用植物的树皮和果实的丙酮提取物含有具有潜在抗诱变/抗癌潜力的成分，可进一步研究。
