Grover I S, Bala S
Department of Botanical Sciences, Guru Nanak Dev University, Amritsar, India.
Indian J Exp Biol. 1992 Apr;30(4):339-41.
Antimutagenicity of water and chloroform extracts of dried myroblan Terminalia chebula was determined against two direct acting mutagens, sodium azide and 4-nitro-o-phenylenediamine (NPD) in strains TA100 and TA1535, and TA97a and TA98 of Salmonella typhimurium respectively and S9-dependent mutagen 2-aminofluorene (2-AF) in TA97a, TA98 and TA100 strains. Water extract reduced NPD as well as 2-AF induced his+ revertants significantly but did not have any perceptible effect against sodium azide included his+ revertants in TA100 and TA1535 strains of S. typhimurium. The pre-incubation studies, where the extract was incubated at 37 degrees C for 30 min with the said mutagen prior to plating, enhanced the inhibitory effect. Autoclaving the water extract reduced the inhibitory effect but the reduction in the effect was not significant. No inhibitory effect was observed in any of the strains and against any of the test mutagens with chloroform extract.
分别在鼠伤寒沙门氏菌TA100和TA1535菌株以及TA97a和TA98菌株中，测定了诃子干水提取物和氯仿提取物对两种直接作用诱变剂叠氮化钠和4-硝基邻苯二胺（NPD）的抗诱变性，以及在TA97a、TA98和TA100菌株中对S9依赖性诱变剂2-氨基芴（2-AF）的抗诱变性。水提取物显著降低了NPD以及2-AF诱导的组氨酸营养缺陷型回复突变菌落数，但对鼠伤寒沙门氏菌TA100和TA1535菌株中叠氮化钠诱导的组氨酸营养缺陷型回复突变菌落数没有明显影响。预孵育研究中，提取物在接种前于37℃与上述诱变剂孵育30分钟，增强了抑制作用。对水提取物进行高压灭菌降低了抑制作用，但作用的降低并不显著。氯仿提取物在任何菌株中对任何测试诱变剂均未观察到抑制作用。
