Simpson K W, Fyfe J, Cornetta A, Sachs A, Strauss-Ayali D, Lamb S V, Reimers T J
Department of Clinical Science, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
J Vet Intern Med. 2001 Jan-Feb;15(1):26-32. doi: 10.1892/0891-6640(2001)015<0026:scoscv>2.3.co;2.
The present study sought to determine the spectrum of diseases associated with subnormal concentrations of serum cobalamin in cats undergoing investigation of suspected gastrointestinal problems. The solid-phase boil radioassay (RA) for cobalamin employed in the present study was immunologically specific, precise, and accurate, with a sensitivity of 15 pg/mL. The RA yielded results that strongly correlated with those obtained by bioassay (Spearmann rho = .805; P < .0001), although the absolute values were lower for the RA. Forty-nine of 80 serum samples submitted during the period of January 1996-January 1998 had cobalamin concentrations below the reference range for healthy cats (range 900-2,800 pg/mL; mean +/- SD, 1,775 +/- 535 pg/mL; n = 33). Cats with subnormal cobalamin concentrations (mean +/- SD; 384 +/- 272 pg/mL, range 3-883 pg/mL) were middle-aged or older and were presented for weight loss. diarrhea, vomiting, anorexia, and thickened intestines. Definitive diagnoses in 22 cats included inflammatory bowel disease (IBD), intestinal lymphoma, cholangiohepatitis or cholangits, and pancreatic inflammation. Serum concentrations of cobalamin were particularly low in cats with intestinal lymphoma, three-fifths of whom also had subnormal serum concentrations of folate (< 9 ng/mL). The simultaneous presence of disease in the intestines, pancreas, or hepatobiliary system in many cats made it difficult to determine the cause of subnormal cobalamin concentrations. The circulating half-life of parenteral cyanocobalamin was shorter in 2 cats with IBD (5 days) than in 4 healthy cats (12.75 days). The presence of subnormal serum concentrations of cobalamin in 49 of 80 cats evaluated suggests that the measurement of serum cobalamin may be a useful indirect indicator of enteric or pancreatic disease in cats. The rapid depletion of circulating cobalamin in cats suggests that cats may be highly susceptible to cobalamin deficiency. However, the relationship of subnormal serum cobalamin concentrations to cobalamin deficiency and the effect of cobalamin deficiency on cats remain to be determined.
本研究旨在确定在接受疑似胃肠道问题检查的猫中，与血清钴胺素浓度低于正常水平相关的疾病谱。本研究中采用的固相煮沸放射分析法（RA）检测钴胺素具有免疫特异性、精确性和准确性，灵敏度为15 pg/mL。尽管RA得到的绝对值较低，但其结果与生物测定法得到的结果高度相关（斯皮尔曼相关系数ρ = 0.805；P < 0.0001）。在1996年1月至1998年1月期间提交的80份血清样本中，有49份的钴胺素浓度低于健康猫的参考范围（范围为900 - 2800 pg/mL；平均值±标准差，1775±535 pg/mL；n = 33）。钴胺素浓度低于正常水平的猫（平均值±标准差；384±272 pg/mL，范围为3 - 883 pg/mL）为中年或老年猫，表现为体重减轻、腹泻、呕吐、厌食和肠道增厚。22只猫的确诊疾病包括炎症性肠病（IBD）、肠道淋巴瘤、胆管肝炎或胆管炎以及胰腺炎。肠道淋巴瘤猫的血清钴胺素浓度特别低，其中五分之三的猫血清叶酸浓度也低于正常水平（< 9 ng/mL）。许多猫同时存在肠道、胰腺或肝胆系统疾病，这使得难以确定钴胺素浓度低于正常水平的原因。2只患有IBD的猫中，肠胃外氰钴胺的循环半衰期（5天）比4只健康猫（12.75天）短。在接受评估的80只猫中，有49只血清钴胺素浓度低于正常水平，这表明检测血清钴胺素可能是猫肠道或胰腺疾病的有用间接指标。猫体内循环钴胺素的快速消耗表明猫可能对钴胺素缺乏高度敏感。然而，血清钴胺素浓度低于正常水平与钴胺素缺乏的关系以及钴胺素缺乏对猫的影响仍有待确定。
