Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA.
Department of Population Health and Reproduction, 100K Pathogen Genome Project, University of California School of Veterinary Medicine, University of California, Davis, Davis, CA, USA.
Sci Rep. 2024 Mar 23;14(1):6939. doi: 10.1038/s41598-024-57004-2.
Chronic enteropathies (CE) are common disorders in cats and the differentiation between the two main underlying diseases, inflammatory bowel disease (IBD) and low-grade intestinal T-cell lymphoma (LGITL), can be challenging. Characterization of the serum metabolome could provide further information on alterations of disease-associated metabolic pathways and may identify diagnostic or therapeutic targets. Unbiased metabolomics analysis of serum from 28 cats with CE (14 cats with IBD, 14 cats with LGITL) and 14 healthy controls identified 1,007 named metabolites, of which 129 were significantly different in cats with CE compared to healthy controls at baseline. Random Forest analysis revealed a predictive accuracy of 90% for differentiating controls from cats with chronic enteropathy. Metabolic pathways found to be significantly altered included phospholipids, amino acids, thiamine, and tryptophan metabolism. Several metabolites were found to be significantly different between cats with IBD versus LGITL, including several sphingolipids, phosphatidylcholine 40:7, uridine, pinitol, 3,4-dihydroxybenzoic acid, and glucuronic acid. However, random forest analysis revealed a poor group predictive accuracy of 60% for the differentiation of IBD from LGITL. Of 129 compounds found to be significantly different between healthy cats and cats with CE at baseline, 58 remained different following treatment.
慢性肠病（CE）在猫中很常见，区分两种主要的潜在疾病，炎症性肠病（IBD）和低级别肠道 T 细胞淋巴瘤（LGITL），具有挑战性。血清代谢组学的特征可提供有关疾病相关代谢途径改变的更多信息，并可能确定诊断或治疗靶点。对 28 只患有 CE 的猫（14 只患有 IBD，14 只患有 LGITL）和 14 只健康对照的血清进行无偏代谢组学分析，确定了 1007 个命名代谢物，其中 129 个在患有 CE 的猫与健康对照之间在基线时存在显着差异。随机森林分析显示，区分对照组和患有慢性肠病的猫的预测准确性为 90％。发现显着改变的代谢途径包括磷脂，氨基酸，硫胺素和色氨酸代谢。与 IBD 相比，在 IBD 猫与 LGITL 猫之间发现了几种代谢物显着不同，包括几种神经酰胺，磷脂酰胆碱 40：7，尿苷，肌醇，对羟基苯甲酸和葡萄糖醛酸。然而，随机森林分析显示，IBD 与 LGITL 的区分的组预测准确性很差，为 60％。在健康猫与患有 CE 的猫之间在基线时发现的 129 种显着不同的化合物中，有 58 种在治疗后仍存在差异。
