Månsson P E, Holmquist L, Deutsch A, Nordén A
Scand J Haematol. 1975 Mar;14(1):42-56. doi: 10.1111/j.1600-0609.1975.tb00292.x.
Rapidly labelled high molecular weight nuclear RNA from lymphocytes of chronic lymphocytic leukaemia was analysed for ribonuclease-stable adenylate-rich and double-stranded regions. The polyadenylate content corresponds to 0.4-0.5 percent and the content of double-stranded sequences to 2-4 percent of the total nucleotides. Partial association of polyadenylate segments with double-stranded regions was found by comparative analysis of (3H)-adenosine and (3H)-uridine labelled ribonuclease-stable RNA before and after thermal denaturation. Comparison with normal lymphocytes shows lower proportions of polyadenylate-containing RNA binding to poly(U)-Sepharose in leukaemia cells than in normals. Partial degradation of rapidly labelled high molecular weight RNA was found in leukaemia cases with low white cell counts.
对慢性淋巴细胞白血病淋巴细胞中快速标记的高分子量核RNA进行分析，以检测核糖核酸酶稳定的富含腺苷酸区域和双链区域。聚腺苷酸含量相当于总核苷酸的0.4 - 0.5%，双链序列含量为2 - 4%。通过对热变性前后用（3H）-腺苷和（3H）-尿苷标记的核糖核酸酶稳定RNA进行比较分析，发现聚腺苷酸片段与双链区域存在部分关联。与正常淋巴细胞相比，白血病细胞中与聚（U）-琼脂糖结合的含聚腺苷酸RNA比例更低。在白细胞计数低的白血病病例中发现了快速标记的高分子量RNA的部分降解。
