Mänsson P E, Deutsch A, Brandt L
Scand J Haematol. 1976 Oct;17(4):276-84. doi: 10.1111/j.1600-0609.1976.tb01185.x.
Rapidly labelled high molecular weight heterogeneous nuclear RNA (Hn RNA) from normal and chronic lymphocytic leukaemia (CLL) lymphocytes has been fractionated into two classes by chromatography on poly (U) sephasose. The (+) Hn RNA is bound to poly (U) sepharose and contains both a long poly (A) segment (100-200 nucleotides) and shorter (A) rich sequences (approximately 20-30 nucleotides. (-) Hn RNA is not bound to poly (U) sepharose and lacks the longer poly (A) segment but does contain shorter (A) rich sequences. Partial association of the short (A) rich segments with double-stranded regions was found for both classes of Hn RNA but was most pronounced in (-) Hn RNA from high WBC cases of CLL. Both classes of Hn RNA from CLL lymphocytes contain varying but generally higher amounts of the short (A) rich fraction than those in normals. Similar amounts of the longer poly (A) segments were found in (+) Hn RNA from both CLL and normal lymphocytes.
来自正常淋巴细胞和慢性淋巴细胞白血病（CLL）淋巴细胞的快速标记的高分子量不均一核RNA（Hn RNA），通过在聚（U）琼脂糖上进行层析，已被分离为两类。（+）Hn RNA与聚（U）琼脂糖结合，包含一个长的聚（A）片段（100 - 200个核苷酸）和较短的富含（A）的序列（约20 - 30个核苷酸）。（-）Hn RNA不与聚（U）琼脂糖结合，缺乏较长的聚（A）片段，但确实含有较短的富含（A）的序列。对于两类Hn RNA，都发现短的富含（A）的片段与双链区域有部分关联，但在CLL高白细胞病例的（-）Hn RNA中最为明显。来自CLL淋巴细胞的两类Hn RNA都含有数量各异但通常比正常淋巴细胞中更多的短的富含（A）的部分。在CLL淋巴细胞和正常淋巴细胞的（+）Hn RNA中发现了相似数量的较长聚（A）片段。
