通过抗F1荚膜抗原特异性IgG/IgM酶联免疫吸附测定和免疫印迹法进行人类鼠疫的血清学诊断。
Serodiagnosis of human plague by an anti-F1 capsular antigen specific IgG/IgM ELISA and immunoblot.
作者信息
Neubauer H, Rahalison L, Brooks T J, Aleksic S, Chanteau S, Splettstösser W D
机构信息
Institut für Mikrobiologie, Sanitätsakademie der Bundeswehr, München, Germany.
出版信息
Epidemiol Infect. 2000 Dec;125(3):593-7. doi: 10.1017/s0950268800004647.
Plague is a re-emerging disease endemic in at least 24 countries. Non-endemic countries should be able to confirm plague to prevent outbreaks due to imported cases. We established a combination of a IgG/IgM screening ELISA and a confirmation immunoblot employing F1 capsular antigen (CA) for the serodiagnosis of plague in countries where yersiniosis is present. The ELISA and the immunoblot assay showed a specificity of 96.1% and 100% among sera from healthy German blood donors. This group had a seroprevalence of 39% of anti-yersinia outer protein (YOP) antibodies obviously caused by previous Y. enterocolitica infection. The ELISA detected anti-F1 CA antibodies in 22 and the immunoblot in 20 out of 26 sera of plague vaccinees. Five control sera from bacteriologically confirmed plague cases from Madagascar reacted positively. It can be concluded that anti-YOP antibodies do not affect assays based on purified F1 CA.
鼠疫是一种在至少24个国家流行的再度出现的疾病。非流行国家应能够确诊鼠疫，以预防因输入性病例导致的疫情爆发。我们建立了一种IgG/IgM筛查酶联免疫吸附测定（ELISA）和一种采用F1荚膜抗原（CA）的确认免疫印迹法，用于在存在耶尔森菌病的国家进行鼠疫的血清学诊断。ELISA和免疫印迹测定在德国健康献血者的血清中显示出96.1%和100%的特异性。该组人群抗耶尔森菌外膜蛋白（YOP）抗体的血清阳性率为39%，显然是由先前的小肠结肠炎耶尔森菌感染引起的。在26名鼠疫疫苗接种者的血清中，ELISA检测到22份血清中有抗F1 CA抗体，免疫印迹法检测到20份血清中有抗F1 CA抗体。来自马达加斯加经细菌学确诊的5份鼠疫病例对照血清呈阳性反应。可以得出结论，抗YOP抗体不影响基于纯化F1 CA的检测方法。
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