Sewell D L, Wostmann B S, Gairola C, Aleem M I
Am J Physiol. 1975 Feb;228(2):526-9. doi: 10.1152/ajplegacy.1975.228.2.526.
The ADP:O ratios and State 3 (ADP-stimulated) and State 4 (controlled) rates of succinate, beta-hydroxybutyrate, isocitrate, glutamate, pyruvate + malate, alpha-ketoglutarate, and ascorbate + N,N,N',N'-tetramethylphenylenediamine (TMPD) oxidation were examined in liver mitochondria from germ-free and conventional rats of both Lobund Wistar (100-day-old) and Fisher (120-day-old) strains. The State 3 respiration rates of isolated mitochondria from germ-free and conventional rats were comparable except for the rate of succinate oxidation in the Wistar rats, which was significantly lower (approx. 10%). The State 4 respiration rates were generally lower in mitochondria isolated from germ-free Fisher rats (approx. 8%) and significantly lower (approx. 18%) in germ-free Wistar rats. The ADP:O ratios were similar in germ-free and conventional rats. Serum thyroxine concentrations indicated delayed maturation of thyroid function in young germ-free rats, but adult animals had concentrations similar to those found in conventional rats. The results indicate that, although absence of a microflora results in a 20-30% reduction in metabolic rate, the germ-free state has little influence on the functional respiration or oxidative phosphorylation of mitochondria isolated from the liver of the adult rat.
对来自无菌和普通饲养的Lobund Wistar（100日龄）和Fisher（120日龄）品系大鼠肝脏线粒体中琥珀酸、β-羟基丁酸、异柠檬酸、谷氨酸、丙酮酸+苹果酸、α-酮戊二酸以及抗坏血酸+N,N,N',N'-四甲基对苯二胺（TMPD）的氧化作用的ADP:O比率、状态3（ADP刺激）和状态4（对照）速率进行了检测。无菌和普通饲养大鼠分离出的线粒体的状态3呼吸速率相当，但Wistar大鼠中琥珀酸氧化速率除外，该速率显著较低（约10%）。无菌Fisher大鼠分离出的线粒体的状态4呼吸速率通常较低（约8%），而无菌Wistar大鼠中该速率显著更低（约18%）。无菌和普通饲养大鼠的ADP:O比率相似。血清甲状腺素浓度表明幼年无菌大鼠甲状腺功能成熟延迟，但成年动物的浓度与普通饲养大鼠中的浓度相似。结果表明，虽然缺乏微生物群会导致代谢率降低20 - 30%，但无菌状态对成年大鼠肝脏分离出的线粒体的功能性呼吸或氧化磷酸化影响很小。
