Walker M P, Schlaberg R, Hays A P, Bowser R, Lipkin W I
Department of Neurology, University of California, Irvine 92697-4292, USA.
Ann Neurol. 2001 Feb;49(2):249-53.
The role of enteroviruses in pathogenesis of amyotrophic lateral sclerosis (ALS) is controversial. A recent study, based on reverse transcription-polymerase chain reaction (RT-PCR) analysis of spinal cord, reported identification of a novel echovirus in 15 of 17 French subjects with ALS and only 1 of 29 subjects with other neurologic diseases. We established a real-time RT-PCR method based on this novel echovirus sequence and used this method and that previously employed for analysis of the French subjects to determine the prevalence of echoviral sequences in spinal cord and motor cortex of sporadic ALS subjects from the United States. No echoviral sequences were found in 20 spinal cord and 10 motor cortex samples from autopsy-confirmed cases of ALS or 13 spinal cord and 5 motor cortex samples from subjects with no motor neuron disease.
肠道病毒在肌萎缩侧索硬化症（ALS）发病机制中的作用存在争议。最近一项基于脊髓逆转录聚合酶链反应（RT-PCR）分析的研究报告称，在17名法国ALS患者中的15名以及29名其他神经系统疾病患者中的1名身上鉴定出一种新型艾柯病毒。我们基于这种新型艾柯病毒序列建立了一种实时RT-PCR方法，并使用该方法以及之前用于分析法国患者的方法来确定来自美国的散发性ALS患者脊髓和运动皮层中艾柯病毒序列的流行情况。在20例经尸检确诊的ALS病例的脊髓样本和10例运动皮层样本中，以及在13例脊髓样本和5例无运动神经元疾病患者的运动皮层样本中，均未发现艾柯病毒序列。
