大鼠和小鼠红细胞中的鸟嘌呤氨基水解酶：一种有效的tRNA鸟苷酸化抑制剂。

Guanine aminohydrolase in rat and mouse red cells: a potent inhibitor of guanylation of tRNA.

Farkas W R, Singh R D

Biochim Biophys Acta. 1975 Jan 23;377(1):166-73. doi: 10.1016/0005-2744(75)90297-1.

Abstract

The red blood cells of mice and rats contained guanine aminohydrolase (EC 3.5.4.3). This enzyme was not present in rabbit, sheep or human red blood cells. 2. The enzyme from rat blood cells was separated into two activities by column chromatography on DEAE-cellulose. Both isozymes were labile but it was possible to show that the more abundant enzyme followed Michaelis-Menten kinetics, had an apparent Km of 4.0-10-6 M and was not activated by GTP nor inhibited by allantoin. 3. We believe, therefore, that guanine aminohydrolase was the protein in rat and mouse red blood cells that inhibited the enzyme (in rabbit reticulocytes) responsible for guanylation of tRNA.

摘要

小鼠和大鼠的红细胞含有鸟嘌呤氨基水解酶（EC 3.5.4.3）。这种酶不存在于兔、羊或人类的红细胞中。2. 通过在DEAE - 纤维素上进行柱色谱，将大鼠血细胞中的酶分离为两种活性。两种同工酶都不稳定，但有可能表明较丰富的那种酶遵循米氏动力学，表观Km为4.0×10⁻⁶ M，且不受GTP激活，也不受尿囊素抑制。3. 因此，我们认为鸟嘌呤氨基水解酶是大鼠和小鼠红细胞中抑制（兔网织红细胞中的）负责tRNA鸟苷酸化的酶的蛋白质。