Farkas W R, Chernoff D
Nucleic Acids Res. 1976 Oct;3(10):2521-8. doi: 10.1093/nar/3.10.2521.
Two of the tRNA's found in rabbit reticulocytes are substrates for a post-transcriptional modification leading to the incorporation of guanine into the polynucleotide chain. The major guanylated tRNA was previously identified as tRNA (His). In the present report we show that the minor guanylated tRNA is tRNA (Asn), and that just as in the case of tRNA (His), the guanine is located in an internal position. There are only two tRNA (Asn) in reticulocytes. We further show that one of these, the one that is not labeled with guanine, contains the hypermodified base known as Q. tRNA (Asn) does not contain Q.
在兔网织红细胞中发现的两种转运核糖核酸(tRNA)是转录后修饰的底物,这种修饰导致鸟嘌呤掺入多核苷酸链中。主要的鸟苷酸化tRNA先前被鉴定为tRNA(His)。在本报告中,我们表明次要的鸟苷酸化tRNA是tRNA(Asn),并且正如tRNA(His)的情况一样,鸟嘌呤位于内部位置。网织红细胞中只有两种tRNA(Asn)。我们进一步表明,其中一种未被鸟嘌呤标记的tRNA含有称为Q的超修饰碱基。tRNA(Asn)不含有Q。