Vázquez Blanco E, López Mahía P, Muniategui Lorenzo S, Prada Rodríguez D, Fernández Fernández E
Department of Analytical Chemistry, University of La Coruña, Spain.
Fresenius J Anal Chem. 2000 Feb;366(3):283-8. doi: 10.1007/s002160050054.
Microwave energy was applied to extract polycyclic aromatic hydrocarbons (PAHs) and linear aliphatic hydrocarbons (LAHs) from marine sediments. The influence of experimental conditions, such as different extracting solvents and mixtures, microwave power, irradiation time and number of samples extracted per run has been tested using real marine sediment samples; volume of the solvent, sample quantity and matrix effects were also evaluated. The yield of extracted compounds obtained by microwave irradiation was compared with that obtained using the traditional Soxhlet extraction. The best results were achieved with a mixture of acetone and hexane (1:1), and recoveries ranged from 92 to 106%. The extraction time is dependent on the irradiation power and the number of samples extracted per run, so when the irradiation power was set to 500 W, the extraction times varied from 6 min for 1 sample to 18 min for 8 samples. Analytical determinations were carried out by high-performance liquid chromatography (HPLC) with an ultraviolet-visible photodiode-array detector for PAHs and gas chromatography (GC) using a FID detector for LAHs. To test the accuracy of the microwave-assisted extraction (MAE) technique, optimized methodology was applied to the analysis of standard reference material (SRM 1941), obtaining acceptable results.
采用微波能量从海洋沉积物中提取多环芳烃（PAHs）和直链脂肪烃（LAHs）。使用真实的海洋沉积物样品测试了不同萃取溶剂及其混合物、微波功率、辐照时间和每次运行萃取样品数量等实验条件的影响；还评估了溶剂体积、样品量和基质效应。将微波辐照获得的萃取化合物产率与传统索氏萃取法获得的产率进行了比较。使用丙酮和己烷（1:1）的混合物取得了最佳结果，回收率在92%至106%之间。萃取时间取决于辐照功率和每次运行萃取的样品数量，因此当辐照功率设定为500 W时，萃取时间从1个样品的6分钟到8个样品的18分钟不等。采用高效液相色谱（HPLC）配备紫外 - 可见光电二极管阵列检测器分析PAHs，采用气相色谱（GC）配备FID检测器分析LAHs。为测试微波辅助萃取（MAE）技术的准确性，将优化方法应用于标准参考物质（SRM 1941）的分析，获得了可接受的结果。
