Pufal E, Sykutera M, Rochholz G, Schütz H W, Sliwka K, Kaatsch H J
Katedra i Zaklad Medycyny Sadowej, Akademia Medyczna im. Ludwika Rydygiera, Bydgoszcz, Poland.
Fresenius J Anal Chem. 2000 Jul;367(6):596-9. doi: 10.1007/s002160000420.
A solid-phase extraction method routinely used for serum samples was improved and applied to the qualitative and quantitative determination of paracetamol in different body fluids, e.g. blood, urine, cerebrospinal fluid, synovial fluid, vitreous humor, and in tissue samples. A very simple method showed best results: Body fluids were mixed with phenacetine as internal standard and phosphate buffer (pH 6.8). Then protein was precipitated using acetonitrile. After strong centrifugation the supematant was transferred to a preconditioned Bakerbond C18-SPE-column. Elution with methanol without a prior washing step showed best recovery rates. The extracts were investigated using high-performance liquid chromatography with ultraviolet detection, a photometrical and an immunochemical method.
一种常规用于血清样本的固相萃取方法得到改进，并应用于不同体液（如血液、尿液、脑脊液、滑液、玻璃体液）以及组织样本中对乙酰氨基酚的定性和定量测定。一种非常简单的方法显示出最佳结果：将体液与非那西丁作为内标以及磷酸盐缓冲液（pH 6.8）混合。然后用乙腈沉淀蛋白质。经过强力离心后，将上清液转移至预处理过的Bakerbond C18-SPE柱。在没有预先洗涤步骤的情况下用甲醇洗脱显示出最佳回收率。使用带有紫外检测的高效液相色谱、光度法和免疫化学方法对提取物进行研究。
