Wong L T, Solomonraj G, Thomas B H
J Pharm Sci. 1976 Jul;65(7):1064-6. doi: 10.1002/jps.2600650728.
A method for the rapid estimation of free acetaminophen in biological fluids is described. The assay involves either extraction and high-pressure liquid chromatographic analysis on a 10-mum particle-size silica gel column, using a mobile phase of 10% chloroform in tetrahydrofuran. The procedure was used to determine acetaminophen levels in urine from two healthy volunteers who ingested 650 mg of 14C-acetaminophen (20 muCi), and the accuracy of the method was compared with the carbon-14 determination. The limit of detectability for acetaminophen is 1 mug/ml.
本文描述了一种快速估算生物体液中游离对乙酰氨基酚的方法。该测定方法包括提取，然后在粒径为10μm的硅胶柱上进行高压液相色谱分析，流动相为四氢呋喃中10%的氯仿。该程序用于测定两名健康志愿者摄入650mg 14C-对乙酰氨基酚（20μCi）后的尿液中对乙酰氨基酚水平，并将该方法的准确性与碳-14测定法进行比较。对乙酰氨基酚的检测限为1μg/ml。
