Douidar S M, Ahmed A E
J Clin Chem Clin Biochem. 1982 Nov;20(11):791-8. doi: 10.1515/cclm.1982.20.11.791.
A rapid and sensitive high performance liquid chromatographic method was developed for separation and quantitation of acetaminophen, salicylic acid and salicyluric acid in human plasma and urine. The method involved ethyl acetate extraction of the three drugs from plasma or urine followed by evaporation of the organic solvent phase and dissolution of the residue in 100 microliters methanol. A 20 microliters aliquot was analysed on a reversed phase column using an isocratic system of 60 ml/l acetonitrile in 4 mmol/l phosphate buffer, pH 5.7 as a mobile phase and a variable wave length UV detector set at 237 nm. The procedure was used to determine the amounts of the three compounds in plasma and urine of two healthy volunteers who ingested 650 mg of aspirin followed one hour later by 650 mg of acetaminophen.
建立了一种快速灵敏的高效液相色谱法，用于分离和定量测定人血浆和尿液中的对乙酰氨基酚、水杨酸和水杨尿酸。该方法包括用乙酸乙酯从血浆或尿液中萃取这三种药物，然后蒸发有机溶剂相，将残留物溶解于100微升甲醇中。取20微升等分试样，在反相柱上进行分析，使用由60毫升/升乙腈与4毫摩尔/升pH 5.7的磷酸盐缓冲液组成的等度洗脱系统作为流动相，并采用设定在237纳米的可变波长紫外检测器。该方法用于测定两名健康志愿者血浆和尿液中三种化合物的含量，这两名志愿者先服用650毫克阿司匹林，一小时后再服用650毫克对乙酰氨基酚。
