Hu Q, Zhou T, Zhang L, Li H, Fang Y
Department of Chemistry, East China Normal University, Shang hai.
Fresenius J Anal Chem. 2000 Dec;368(8):844-7. doi: 10.1007/s002160000571.
A simple, reliable and reproducible method, based on capillary zone electrophoresis with amperometric detection, has been developed for the determination of idarubicin in human urine. A carbon disk electrode was used as working electrode. The optimal conditions of separation and detection were pH 5.6 phosphate buffer (0.20 mol/L), 22 kV for the separation voltage and 1.00 V (vs. Ag/AgCl, 3 mol/L KCl) for the detection potential. The linear range was from 4.0 x 10(-7) to 2.0 x 10(-5) mol/L with a regression coefficient of 0.9986, and the detection limit was 8.0 x 10(-8) mol/L. The method was directly applied to the determination of idarubicin in spiked human urine without any other sample pretreatment except filtration, and the assay results were satisfactory.
已开发出一种基于毛细管区带电泳-安培检测的简单、可靠且可重复的方法，用于测定人尿液中的伊达比星。使用碳盘电极作为工作电极。分离和检测的最佳条件为：pH 5.6的磷酸盐缓冲液（0.20 mol/L），分离电压22 kV，检测电位1.00 V（相对于Ag/AgCl，3 mol/L KCl）。线性范围为4.0×10⁻⁷至2.0×10⁻⁵ mol/L，回归系数为0.9986，检测限为8.0×10⁻⁸ mol/L。该方法直接应用于加标人尿液中伊达比星的测定，除过滤外无需任何其他样品预处理，测定结果令人满意。
