Navarro F A, Stoner M L, Lee H B, Park C S, Wood F M, Orgill D P
Brigham and Women's Hospital, Boston, Massachusetts 02115-6195, USA.
J Burn Care Rehabil. 2001 Jan-Feb;22(1):41-6. doi: 10.1097/00004630-200101000-00009.
Melanocyte restoration is critical in reconstituting skin color. We developed a spotted (piebald) pig wound model to study methods of restoring melanocytes to the epidermis. Paired, full-thickness, porcine wounds were covered with nonpigmented, fully expanded, 3:1 meshed, split-thickness skin grafts and were sprayed with an epidermal cell suspension. The suspensions were highly pigmented skin (HPS) cell isolates for half of the wounds (n = 16) and nonpigmented skin (NPS) cell isolates for the remaining wounds (n = 16). Histologic sections showed 6.0 +/- 3.0 and 15 +/- 4.0 pigmented melanocytes per high-power field on days 8 and 20 in HPS-treated wounds and no pigmented melanocytes in NPS-treated wounds. Melanin pigment was dispersed in all layers of the epithelium for the HPS group on day 20 compared with a lack of melanin pigment observed in the NPS group. Cell spraying may provide a clinical method to restore color to skin; further work is needed to control the expression of melanin.
黑素细胞的恢复对于重建皮肤颜色至关重要。我们开发了一种花斑猪伤口模型，以研究将黑素细胞恢复至表皮的方法。将成对的全层猪伤口用无色素、充分扩张的3:1网状中厚皮片覆盖，并喷洒表皮细胞悬液。对于一半的伤口（n = 16），悬液为高色素皮肤（HPS）细胞分离物，其余伤口（n = 16）则为无色素皮肤（NPS）细胞分离物。组织学切片显示，在HPS处理的伤口中，第8天和第20天时每高倍视野分别有6.0±3.0和15±4.0个色素沉着的黑素细胞，而在NPS处理的伤口中未发现色素沉着的黑素细胞。与NPS组中未观察到黑色素不同，HPS组在第20天时黑色素分散在上皮的所有层中。细胞喷洒可能为恢复皮肤颜色提供一种临床方法；需要进一步开展工作来控制黑色素的表达。
