Hackl Florian, Kiwanuka Elizabeth, Philip Justin, Gerner Philipp, Aflaki Pejman, Diaz-Siso J Rodrigo, Sisk Geoffroy, Caterson E J, Junker Johan P E, Eriksson Elof
Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, United States.
Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, United States.
Burns. 2014 Mar;40(2):274-80. doi: 10.1016/j.burns.2013.06.002. Epub 2013 Jul 6.
Transplantation of skin micrografts in a 1:100 ratio regenerate the epidermis of full-thickness wounds in pigs within 14 days in a wet environment. The aim of the current study was to combine micrografts and commercially available moist dressings. We hypothesized that micrografts regenerate the epidermis when covered with a moist dressing. 5cm×5cm and 10cm×10cm full-thickness wounds were created on the backs of pigs. Wounds were transplanted with 0.8mm×0.8mm micrografts created from a split-thickness skin graft in a 1:100 ratio. 5cm×5cm wounds were treated with wound chambers, moist dressings or dry gauze (non-transplanted control group). 10cm×10cm wounds were compared to non-transplanted wounds, both covered with moist dressings. Reepithelialization was assessed in biopsies from day 10, 14 and 18 post-transplantation. 5cm×5cm transplanted wounds covered with moist dressings showed 69.5±20.6% reepithelialization by day 14 and 90.5±10.4% by day 18, similar to wounds covered with a wound chamber (63.9±16.7 and 86.2±11.9%, respectively). 18 days post-transplantation, 10cm×10cm transplanted wounds covered with moist dressings showed 66.1±10.3% reepithelialization, whereas nontransplanted wounds covered with moist dressings were 40.6±6.6% reepithelialized. We conclude that micrografts combined with clinically available moist dressings regenerate the epidermis of full-thickness wounds.
以1:100的比例移植皮肤微移植片，可在14天内在潮湿环境中使猪的全层伤口表皮再生。本研究的目的是将微移植片与市售的湿性敷料相结合。我们假设微移植片在覆盖湿性敷料时可使表皮再生。在猪的背部制造5cm×5cm和10cm×10cm的全层伤口。用从断层皮片中制备的0.8mm×0.8mm微移植片以1:100的比例移植到伤口上。5cm×5cm的伤口分别用伤口腔室、湿性敷料或干纱布治疗（非移植对照组）。将10cm×10cm的伤口与同样覆盖湿性敷料的未移植伤口进行比较。在移植后第10天、14天和18天的活检中评估再上皮化情况。到第14天，覆盖湿性敷料的5cm×5cm移植伤口的再上皮化率为69.5±20.6%，到第18天为90.5±10.4%，与覆盖伤口腔室的伤口相似（分别为63.9±16.7%和86.2±11.9%）。移植后18天，覆盖湿性敷料的10cm×10cm移植伤口的再上皮化率为66.1±10.3%，而覆盖湿性敷料的未移植伤口的再上皮化率为40.6±6.6%。我们得出结论，微移植片与临床可用的湿性敷料相结合可使全层伤口的表皮再生。
